Attenuation of inflammation in streptozotocin-induced diabetic rabbits by Matricaria chamomilla oil: A focus on targeting NF- κ B and NLRP3 signaling pathways
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Abstract:
Objectives: The present study was conducted to investigate the protective effects of chamomile oil from Matricaria chamomilla against type 1 diabetes mellitus (T1DM) and its potential mechanisms. Methods: T1DM was established in male New Zealand white rabbits via a single intraperitoneal infusion of streptozotocin (STZ) (80 mg/kg body weight ?1 , dissolved in 0.2 mL of normal saline). Different doses of chamomile oil (25, 50 and 100 mg/kg) were orally administrated to STZ induced diabetic rabbits for 21 consecutive days. The expression of pro-inflammatory cytokines was determined using ELISA assay. The expression of NF- κ B and NLRP3 was measured using Western blot assay. Results: Compared with normal rabbits, STZ-induced diabetic rabbits exhibited significant increased levels of blood glucose and decreased levels of serum insulin that were reversed using middle and high tested dose of chamomile oil. Likewise, STZ-induced diabetic rabbits showed a significant increased expression of NF- κ B and NLRP3 proteins in the pancreas tissue that was reversed by high tested dose of chamomile oil. Conclusion: Collectively, our findings demonstrated that chamomile oil possesses anti-hyperglycemic, and anti-inflammatory activities in STZ-induced diabetic rabbits by targeting inflammatory cytokines and NF- κ B and NLRP3 signaling pathways.
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This study was financially supported by a research grant (Grant no. 77004821) from the Phytochemistry Research Center of Shahid Beheshti University of Medical Sciences.
Saeid Saghahazrati a, Seyed Abdulmajid Ayatollahi a, b,c, Farzad Kobarfard a, d, Bagher Minaii Zang e. Attenuation of inflammation in streptozotocin-induced diabetic rabbits by Matricaria chamomilla oil: A focus on targeting NF- κ B and NLRP3 signaling pathways[J]. Chinese Herbal Medicines (CHM),2020,12(1):73-78
