Objective To identify the therapeutic effect and possible mechanisms of Chinese medicine Sanqi Tongshuan Tablets (SQTS) on sequelae post-stroke in rats. Methods The rat cerebral ischemia sequelae post-stroke models were successfully induced by blocking the middle cerebral artery with electric coagulator after the seventh week and balance beam test ≤4. The rats were then received with SQTS (0.5, 1, and 2 g/kg) and Naodesheng (NDS, 1.24 g/kg), Vinpocetine (VP, 1.55 mg/kg) for 30 d. The beam-walking test and shuttle test were performed before and after 10, 20, and 30 d of administration. In addition, histopathology changes and GAP-43, GFAP were measured by H&E staining and immunohistochemisty. Results The model displayed signs of brain damage on motor function, learning and memory function and histopathology. After 30 d of treatment, SQTS at different dose (0.5, 1.0, and 2.0 g/kg) restored the beam-walking scores by 21.7% (P > 0.05), 30.4% (P > 0.05), and 39.1% (P < 0.05); Decreased electric shock by 35.0% (P > 0.05), 50.0% (P > 0.05), and 75.0% (P < 0.05), respectively. On the other hand, the histological changes were less severe and the GAP-43 expression increased in hippocampal CA1 and cortical region. Conclusion SQTS showed therapeutic benefits on sequelae post-stroke in rats, which might be through the pathway of regeneration or neuroplasticity.
关键词:
学习记忆;运动功能;大鼠;三七通栓片;中风后遗症
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This work was supported by Tianjin 131 Innovative Talent Training Project (2016).
