Objective To evaluate the effect of high concentration of chlorogenic acid (CGA) on non-alcoholic fatty liver disease (NAFLD) in normal and oleic acid (OA) treated HepG2 cells, as well as the underlying mechanism involved in the fat accumulation, oxidative stress and insulin resistance (IR) induced by CGA treatment. Methods OA (0.5 mmol/L) induced hepatic steatosis was established in HepG2 cells as an in vitro model of NAFLD. The normal and OA-treated HepG2 cells were treated by CGA (0, 0.5, 1, and 2 mmol/L) for 24 h, then cellular lipid droplets, reactive oxygen species (ROS), and glucose uptake were evaluated by Oil Red O staining and cellular biochemical assays, respectively. Signaling pathways involved in?adipogenesis including SREBP-1c and PNPLA3, oxidative stress, and IR including CYP2E1 and CYP4A, were investigated by Western blot and RT-qPCR. Results CGA (0.5, 1, and 2 mmol/L) treatment increased the cellular lipid droplets and the expression of SREBP-1c and PNPLA3 in the tested cells. Additionally, 2-NBDG uptake was significantly increased, whereas the cellular ROS and protein levels of CYP2E1 and CYP4A were significantly decreased in OA-treated cells. Conclusion Our results suggest that high concentrations of CGA ameliorate OA-induced oxidative damage and IR likely by inhibiting the expression of CYP2E1 and CYP4A, promote lipid accumulation by inducing the expression of SREBP-1c and PNPLA3 in the tested cells.
关键词:
绿原酸;糖摄取;HepG2;脂堆积;氧化应激
Keywords:
Project Supported:
Sci-Tech Support Plan of Hubei Province (grant no.2015BCA273); United fund for innovation and entrepreneurship of Ministry of education (201601031006).
Ya-yun Liu, Ting Zhai, Qing-qing Yu, Jing Zhu, Yong Chen. Effect of high exposure of chlorogenic acid on lipid accumulation and oxidative stress in oleic acid-treated HepG2 cells[J]. Chinese Herbal Medicines (CHM),2018,10(2):198-204
