Neuroprotection of Stilbenes from Leaves of Cajanus cajan against Oxidative Damage Induced by Corticosterone and Glutamate in Differentiated PC12 Cells
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摘要:
Abstract:
Objective To investigate the neuroprotective effects of four stilbenes isolated from the leaves of Cajanus cajan. Methods Neuroprotective effects of the four stilbenes were evaluated using rat pheochromocytoma cell line (PC12 cells) damage models induced by corticosterone or glutamate. In order to elaborate whether the neuroprotective effects of stilbenes are related to anti-oxidant properties, both oxidant and anti-oxidant parameters were measured. Results Four stillbenes, namely cajaninstilbene acid (CSA), longistyline A, longistyline C, and cajanolactone A were isolated from the leaves of C. cajan. The results of MTT assay and LDH release assay demonstrated that the four stilbenes possessed neuroprotective effects. Moreover, the treatment on PC12 cells with corticosterone or glutamate could significantly increase the levels of ROS and MDA with accompanying decrease in the activities of SOD and CAT. However, the four tested stilbenes could significantly alleviate such situation by dropping out the levels of ROS and MDA, as well as enhancing the activities of SOD and CAT. Conclusion These results provide a scientific basis for further studies to explore the potential neuroprotective effects on neurodegenerative diseases.
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International Science and Technology Cooperation of China (2011DFA32730, 1108); National Science and Technology Major Project (2012ZX09301002-001)
Ya-min Liu, Sheng-nan Shen, Fang-bo Xia, Qi Chang, Xin-min Liu, Rui-le Pan. Neuroprotection of Stilbenes from Leaves of Cajanus cajan against Oxidative Damage Induced by Corticosterone and Glutamate in Differentiated PC12 Cells[J]. Chinese Herbal Medicines (CHM),2015,7():
