Simultaneous Determination of Four Active Ingredients in Wuji Pellet by HPLC
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
摘要:
Abstract:
Objective To apply the high performance liquid chromatography (HPLC) method to the determination of four ingredients including paeoniflorin, berberine, evodiamine, and rutaecarpin in Wuji Pellet. Methods Using Thermo-ODS2-Hypersil column (250 mm × 4.6 mm, 5 μm) and taking acetonitrile-0.05 mol/L monopotassium phosphate as mobile phase for elution, the conditions were as follows, flow rate of 1.0 mL/min, UV detector wavelength of 225 nm, and column temperature of 25 ℃. Results The standard curves of paeoniflorin, berberine, evodiamine, and rutaecarpine showed a good linear relationship in 0.0124—0.124, 0.0349—0.349 mg/mL, and 0.6—6, 0.5—5 μg/mL. The average recovery rates were 99.45%, 97.69%, 98.18%, and 98.46%, respectively. Conclusion The method is of high sensitivity and nice reproducibility, and it could be used for the simultaneous determination of the four ingredients in Wuji Pellet.
关键词:
Keywords:
Project Supported:
Modernization of Chinese medicine in Guizhou Province (2010-5016)
WANG Qun, LIU Wen, YANG Song, CHEN Da-ye, CHEN Zhong-fen. Simultaneous Determination of Four Active Ingredients in Wuji Pellet by HPLC[J]. Chinese Herbal Medicines (CHM),2013,5(4):297-300
