Objective To investigate the chemical constituents from Aidi Injection. Methods The chemical constituents were isolated by chromatography on Sephadex LH-20 gel columns and reverse phase semi-preparative HPLC repeatedly. Their structures were identified by spectroscopic analysis (NMR and MS). Results Twenty-two compounds were isolated and identified to be 3-O-3′,4′-diacetyl-β-D-xylopyranosyl-6-O-β-D-glucopyranosyl- cycloastragenol (1), astragaloside IV (2), astragaloside II (3), astragaloside I (4), isoastragaloside I (5), acetylastragaloside I (6), ginsenosid Re (7), ginsenoside Rf (8), ginsenoside Rg1 (9), ginsenoside Rb3 (10), notoginsenoside R4 (11), ginsenoside Rb1 (12), ginsenoside Rc (13), ginsenoside Rb2 (14), ginsenoside Rd (15), lucyoside H (16), 3-O-β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl (1→2)-α-L- arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside (17), 3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl [β-D-glucopyranosyl-(1→4)]-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-arabinopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside (18), syringin (19), elentheroside E (20), 4-(1,2,3-trihydroxypropyl)-2,6-dimethoxyphenyl-1-O-β-D-glucopyranoside (21), and coniferin (22). Conclusion Compounds 1－6 are originated from Astragalus membranceus, compounds 7－18 are originated from Panax ginseng, and compounds 19－22 are originated from Acanthopanax senticosus by LC-MS analysis. Compound 1 is a new compound.
ZHANG Miao-miao, LIU Yan-li, CHEN Zhong, LI Xiao-ran, XU Qiong-ming, YANG Shi-lin. A New Triterpenoid Saponin from Aidi Injection[J]. Chinese Herbal Medicines (CHM),2012,4(2):84-86
