Simultaneous Determination of Seven Alkaloids in Fufang Zhenzhu Tiaozhi Capsule by HPLC Coupled with DAD
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摘要:
Abstract:
Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids (magnoflorine, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine) in Fufang Zhenzhu Tiaozhi Capsule. Methods Chromatography was performed on a Dionex Acclaim C18 column (250 mm × 4.6 mm, 5.0 μm) at 30 ℃. The mobile phase was composed of acetonitrile-potassium dihydrogen phosphate solution (0.015 mol/L, 40:60, including 1.7 g/L sodium dodecyl sulfate and phosphoric acid used to regulate pH value to 3.0), with a flow rate of 1.0 mL/min. The detection wavelength was 270 nm. Results The calibration curves of magnoflorine, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine were linear in the range of 1.07－10.65, 0.78－7.55, 0.75－7.50, 1.60－15.95, 2.69－26.85, 2.31－23.10, and 6.04－60.40 mg/mL. The average recoveries of magnoflorine, columbamine, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine were 101.0%, 101.2%, 100.1%, 100.0%, 100.1%, 101.1%, and 99.7%, respectively. Conclusion The method could be used for the quantitative determination of the preparation.
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Cooperation Project in Industry, Education and Research of Guangdong Province and Ministry of Education of China (2009B090300349) and a Cooperation Project of National Science Foundation of Guangdong Province (10351022401000000)
CHEN Yuan-yuan, GUO Jiao, FAN Hui, HUANG Li-hua. Simultaneous Determination of Seven Alkaloids in Fufang Zhenzhu Tiaozhi Capsule by HPLC Coupled with DAD[J]. Chinese Herbal Medicines (CHM),2012,4(3):230-236
