A New Procedure for Separation and Purification of Scutellarin from Erigeron multiradiatus
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摘要:
Abstract:
Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods A proposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus was extracted by ultrasound with 80% methanol. Preliminary separation was performed on macroporous resin column. The performance and adsorption characteristics of three macroporous resins, D140, D141, and D605, were compared and the enrichment procedure was optimized. Further purification was carried out by medium pressure liquid chromatography (MPLC) with ODS column. Results It was demonstrated that D141 had better extractive effects on scutellarin. The MPLC conditions were optimized as follows: 15% ethanol aqueous as mobile phase with flow rate at 2.5 mL/min. The yield and purity of the isolated scutellarin were 1.20 mg/g and 96.5%, respectively. Conclusion The overall procedure is efficient and low-cost, which is considered suitable for the separation and purification of scutellarin from E. multiradiatus. The results provide the scientific basis for developing and using scutellarin in clinic.
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Fundamental Research Funds for Central Universities, Southwest University for Nationalities (09NZYZJ01); National Key Technology R&D Program in the 12th Five Year Plan of China (2012BAI27B07)
ZHANG Zhi-feng, LU Lu-yang, LIU Yuan. A New Procedure for Separation and Purification of Scutellarin from Erigeron multiradiatus[J]. Chinese Herbal Medicines (CHM),2013,5(2):133-137
