Simultaneous Determination of Seven Flavonoids in Aerial Parts of Artemisia frigida by HPLC
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摘要:
Abstract:
Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida. Methods Hypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase, and the detection wavelength was at 283 nm with flow rate at 1 mL/min. Results All calibration curves showed good linear regression (r > 0.9990) within the tested range. All average recovery was more than 98.00% and RSD was less than 3.0% (n = 6). Conclusion The method is steady and with good repeatability, and could be used to determine the content of flavonoids in A. frigida from different areas.
WANG Qing-hu, AO Wu-li-ji, TAI Wen-quan. Simultaneous Determination of Seven Flavonoids in Aerial Parts of Artemisia frigida by HPLC[J]. Chinese Herbal Medicines (CHM),2012,4(3):252-258
