Objective To study the chemical constituents of Rhodiola kirilowii. Methods The compounds were separated and purified by various chromatographic techniques and their structures were elucidated on the basis of physicochemical properties and spectroscopic methods. Results Five compounds were purified and their structures were identified as 4-(β-D-glucopyranosyloxy)-3-hydroxy-2-(hydroxymethyl)-butanenitrile (1), epicatechin (2), arbutin (3), rutin (4), and β-D-glucose (5). Conclusion Compound 1 is a new cyano-compound and other compounds are isolated from the plant for the first time.
