Optimization of Microwave-assisted Extraction of Polyphenols from Enteromorpha prolifra by Orthogonal Test
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摘要:
Abstract:
Objective To optimize microwave-assisted extraction of polyphenols from Enteromorpha prolifra. Methods Based on single-factor tests, an efficient microwave-assisted extraction (MAE) technique was developed to extract bioactive polyphenols from E. prolifra through orthogonal (L16(4)5) test. Results The highest yield (0.923 ± 0.013) mg/g was obtained when microwave power, solvent to raw material ratio, irradiation time, ethanol concentration, and extraction cycles were 500 W, 25 mL/g, 25 min, 40%, and 3, respectively, which was higher than that of Soxhlet extraction with methanol for 6 h, ultrasound-assisted extraction with 40% ethanol for 1 h twice and heat reflux extraction with 40% ethanol for 2 h twice. Conclusion This finding indicates that MAE is a superior technique for the extraction of polyphenols due to less impurity, higher time efficiency and yield.
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Project Supported:
National High Technology Development Project (863 project) (2007AA091701); Zhejiang Provincial Natural Science Foundation (Y2080579); Important Project of Zhejiang Ocean University (21135030107)
LUO Hong-yu, WANG Bin, YU Chun-guang, XU Yin-feng. Optimization of Microwave-assisted Extraction of Polyphenols from Enteromorpha prolifra by Orthogonal Test[J]. Chinese Herbal Medicines (CHM),2010,2(4):321-325
