Objective: To study secondary metabolites from endophytic fungus Colletotruchum sp. HK-08 originated from the leaves of Nerium indicum. Methods: The compounds were isolated by various column chromatographic techniques, and their structures were elucidated by spectroscopic techniques [high resolution electrospray ionization mass spectroscopy (HRESIMS), one-dimensional (1D) and two dimensional (2D) nuclear magnetic resonance spectroscopy (NMR)], as well as comparison with literature data. The Ellman method was used to determine the acetylcholinesterase (AChE) inhibitory activity. Results: Four indole derivatives were identified from Colletotruchum sp. HK-08, including 60-hydroxymonaspiloindole (1), 2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl) acetate (2), 2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate (3), and monaspiloindole (4). Compound 4 presented weak AChE inhibitory activity with IC50 value of (69.30 ± 6.27) μmol/L [tacrine as the positive control, with IC50 value of (0.61 ± 0.07) μmol/L]. Conclusion: Compounds 1–3 were new compounds, and compound 4 had weak AChE inhibitory activity.
关键词:
炭疽菌;植物内生真菌;吲哚衍生物;夹竹桃;次生代谢产物
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Project Supported:
This study was supported by the Hainan Natural Science Foundation (No. 220RC702).
Huiqin Chen a, b,, Hao Zheng a, b,, Caihong Cai a, b, Hao Wang a, b, Cuijuan Gai a, b, Zhiqiong Tan c, Haofu Dai a, b,*, Wenli Mei a, b,*. New indole derivatives from endophytic fungus Colletotruchum sp. HK-08 originated from leaves of Nerium indicum[J]. Chinese Herbal Medicines (CHM),2024,16(2):235-238
