Enzyme-assisted Extraction and Enrichment of Galanthamine from Lycoris aurea
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摘要:
Abstract:
Objective To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L9 (34) orthogonal array design and separation effect of cation exchange resin on galanthamine. Methods Cellulase and pectinase solution was used as the extraction solvent. The effects of pH value of enzyme, amount of complex enzyme, dissociation time, and enzymatic hydrolysis temperature on the extraction results were investigated. Results The optimal conditions were obtained as follows: ratio of solid to liquid (g: mL) 1:10, pH value 4.5, amount of complex enzymes 4%, enzymatic hydrolysis temperature 50 oC, and reaction time 2.0 h. Under these conditions, the extraction yield of galanthamine was 0.0294%. In addition, D-001 cation exchange resin was selected for separation of galanthamine. The separation conditions were that adsorption flow rate was 3 BV/h with reagent of pH 2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia. After separation, the content of galanthamine was increased to 12.31%. Conclusion The results provide a reference for industrial production of galanthamine.
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Hunan Provincial Science and Technology Department (2012TP4002-3); Zhangjiajie Science and Technology Bureau (2014YB17)
Chun-lian Tian, Peng Wang, Ji-xin Qin, Xiao-pan Liu, Ke Song, Zhuo-bing Xiao. Enzyme-assisted Extraction and Enrichment of Galanthamine from Lycoris aurea[J]. Chinese Herbal Medicines (CHM),2016,8(2):182-188
