Bioanalysis and Pharmacokinetics of Eight Active Components from Huanglian Jiedu Decoction in Rat Plasma by LC-ESI-MS/MS Method
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摘要:
Abstract:
Objective To develop a sensitive and rapid liquid chromatography?electrospray ionization?tandem mass spectrometry (LC-ESI-MS/MS) method for the simultaneous determination of wogonin, coptisine, berberine, palmatine, jatrorrhizine, phello- dendrine, magnoflorine, and wogonoside in rat plasma and to evaluate the pharmacokinetic characteristics of Huanglian Jiedu Decoction (HJD). Methods LC separation was performed on an Acquity HSS T3 column (100 mm × 2.1 mm, 1.8 μm) using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water. The detection was accomplished by using positive electrospray ionization in multiple-reaction monitoring mode. Plasma samples were pretreated by protein precipitation. Results The method showed a good linearity over a wide concentration range (r2 > 0.99). The lower limits of quantification were 0.20 ng/mL for coptisine and phellodendrine, 0.48 ng/mL for berberine, 0.10 ng/mL for jatrorrhizine, 0.32 ng/mL for magnoflorine, 0.30 ng/mL for palmatine, and 4.80 ng/mL for wogonin and wogonoside, respectively. The intra- and inter-day precision of the analytes was less than 12.11%, while the accuracy was between ?14.46% and 4.86%. The mean recovery of all the analytes ranged from 93.10% to 110.91%. Conclusion This validated method offers the advantages of high sensitivity. It is successfully applied to evaluating the pharmacokinetic properties of HJD.
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Project Supported:
Program NCET Foundation NSFC (81230090); Global Research Network for Medicinal Plants and King Saud University, Shanghai Leading Academic Discipline Project (B906); Key laboratory of drug research for special environments, PLA, Shanghai Engineering Research Center for the Preparation of Bioactive Natural Products (10DZ2251300); Scientific Foundation of Shanghai China (12401900801, 09DZ1975700, 09DZ1971500, 10DZ1971700); National Major Project of China (2011ZX09307-002-03); National Key Technology R & D Program of China (2012BAI29B06)
Cheng-cheng Peng, Shu-ping Wang, Hui-zi Jin, Jian-fei Tao, Guo-wei Wang, Pan-lei Wei, Xu-feng Zhang, Le Li, Run-hui Liu, Wei-dong Zhang. Bioanalysis and Pharmacokinetics of Eight Active Components from Huanglian Jiedu Decoction in Rat Plasma by LC-ESI-MS/MS Method[J]. Chinese Herbal Medicines (CHM),2014,6(3):198-210
