Smashing Tissue Extraction and HPLC Determination of Paclitaxel and 10-Deacetylbaccatin from Taxus x media
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摘要:
Abstract:
Objective To optimize the extraction technology of Taxus x media by using the contents of Paclitaxel and 10-deacetylbaccatin (10-DAB), two representative active diterpene alkaloids of taxane type from T. x media, as evaluation standard. Methods The smashing tissue extraction (STE) of Paclitaxel and 10-DAB from T. x media, was investigated by comparing with ultrasonic extraction (UE) which was one of the modern technologies of extraction. Results STE was more efficient than UE, and the contents of 10-DAB and Paclitaxel in the extracts obtained by STE were higher than those by UE. Conclusion STE is a fast, high-performance, and energy-saving technology for the extraction of diterpene alkaloids of taxane type. STE also provides a simple, component-safe, workable, and highly efficient method for the extraction of active natural product.
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E&T Modern Center for Natural Products of Liaoning Province of China (2008402021)
LIU Yan-ze, GAO Wen-qin, WANG Ji-wen, ZHANG Yu, ZHAO Yu-qing. Smashing Tissue Extraction and HPLC Determination of Paclitaxel and 10-Deacetylbaccatin from Taxus x media[J]. Chinese Herbal Medicines (CHM),2011,3(3):235-238
