目的 研究草麻黄Ephedra sinica根中的活性成分，并对其进行活性评价。方法 采用硅胶和Sephadex LH-20柱色谱及高效液相进行分离纯化，利用核磁共振、质谱、旋光等谱学技术对化合物进行结构鉴定。选用缺氧诱导建立肺动脉平滑肌细胞恶性增殖模型进行化合物活性筛选。结果 从草麻黄根的95%乙醇提取物中分离得到10个化合物，分别鉴定为7-methoxy-6-methyl-1-naphthoic acid（1）、对二苯酚（2）、对羟基苯甲酸乙酯（3）、原儿茶酸甲酯（4）、原儿茶酸乙酯（5）、苯甲酸（6）、脱氢姜酮（7）、4-氧甲基肉桂酸（8）、3-hydroxy-1-(4-hydroxy-3-methoxyphenyl) propan-1-one（9）、4-羟基-3,5-二甲氧基苯甲醛（10）。结论 化合物1为新的萘类化合物，命名为麻黄根酚H；化合物2～9为首次从草麻黄根中分离得到，化合物5、7为首次从麻黄属植物中分离得到。化合物1～9能显著抑制肺动脉平滑肌细胞的异常增殖。

Objective To study a new naphthalene compound in the roots of Ephedra sinica, the separation and purification, structural identification and activity evaluation of the compound were performed.Methods The secondary metabolites of E. sinica were separated and purified by silica gel, Sephadex LH-20 column chromatography and preparative HPLC. The structures of the isolated compounds were determined by NMR, HR-ESI-MS, polarimetry and etc. In addition, the inhibitory activity against hypoxia-induced abnormal proliferation model of pulmonary arterial smooth muscle cells (PASMCs) was evaluated by MTT assay.Results Ten compounds were isolated and identified as 7-methoxy-6-methyl-1-naphthoic acid (1), p-dihydroxybenzene (2), ethyl p-hydroxybenzoate (3), methyl protocatechuate (4), ethyl protocatechuate (5), benzoic acid (6), dehydrogingerone (7), 4-oxomethylcinnamic acid (8), 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl) propan-1-one (9), and 4-hydroxy-3,5-dimethoxy- benzaldehyde (10).Conclusion Compound 1 was a new arylnaphthalene named ephedphenolic H. Compounds 2—9 were isolated from the roots of E. sinica for the first time, and compounds 5 and 7 were isolated from the genus Ephedra for the first time. The results showed that compounds 1—9 could significantly inhibit the abnormal proliferation of PASMCs.

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国家重点研发计划－中医药现代化研究（2019YFC1708802）；中央引导地方科技发展专项资金（[2016]149）；河南省高层次人才特殊支持计划（ZYQR201810080）。