摘 要：目的 采用超临界CO2 抗溶剂法制备黄芩素微粒，以微粒的体积平均粒径为评价指标，优化制备黄芩素微粒的工

Abstract: Objective Baicalein particles were prepared by using supercritical CO2 anti-solvent technology. With volume average particle size as the evaluation index the preparation process of baicalein was optimized. Methods On the basis of single factor experiments orthogonal experimental design was used to optimize the preparation process of baicalein particles. The experiments such as particle size distribution, scanning electro microscope analysis (SEMA), infrared spectrum (IR) and differential scanning calorimetry (DSC) were used to analyze the particles. Results The orthogonal experimental optimization process conditions were set as follows: solution volumetric flow rate 0.75 mL/min, pressure 8 MPa, temperature 48 ℃ and mass concentration of baicalein 4 mg/mL. Under the optimum conditions, the volume average particle size was obviously smaller than the ingredient. And the shapes of baicalein were irregular. IR and DSC analysis showed that the chemical structure didn't change and the thermodynamic properties of baicalein might transfer. Meanwhile, the purity of baicalein became higher. Conclusion Supercritical CO2 anti-solvent technology was feasible to prepare baicalein particles and it provided a reference basis for preparing ultrafine particles at the same time.

基金项目：江苏省自然科学基金（BK2012763）；大学生创新药物研发能力提高项目“国家级大学生创新创业训练计划项目”（J1030830）