目的 挖掘以芦根为核心的清热解毒药食同源组方并评价其解热作用，开发复方芦根凉茶。方法 对药智网中以芦根为核心的181个中成药及方剂进行数据挖掘，经筛选后得到97个配方数据，对其开展频数统计、聚类与关联规则分析，构建核心复杂网络，得到复方芦根凉茶核心组方，并通过构建干酵母致热大鼠模型研究复方芦根凉茶的解热作用及机制。结果 以芦根为核心的中成药及方剂中，药物性味以甘、寒为主，归肺、胃经，并筛选得到以芦根为核心的8个具有高可信度和较强关联度的中药组合；在中医药理论指导下，将筛选得到的复方芦根凉茶基础方进行加减化裁，确定核心组方为芦根、甘草、薄荷、大枣、麦冬、桔梗、金银花和库尔勒香梨。复方芦根凉茶低、中、高剂量组均能极显著降低发热大鼠体温（P＜0.01），并极显著减少血清肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）含量及下丘脑前列腺素E₂（prostaglandin E₂，PGE₂）、环磷酸腺苷（cyclic adenosine monophosphate，cAMP）含量（P＜0.01）；复方芦根凉茶各剂量能极显著降低血清中白细胞介素-6（interleukin-6，IL-6）含量（P＜0.05、0.01）。结论 复方芦根凉茶能明显降低发热大鼠的体温，其作用机制可能与降低血清中致热因子IL-6、TNF-α含量，减少下丘脑中致热、致炎、致痛因子PGE₂及cAMP的含量从而使体温调定点下移有关。

Objective To explore food and medicine homologous prescriptions for heat-clearing and detoxifying, with Lugen (Phragmitis Rhizoma) as the core, evaluate their antipyretic effect, and develop compound Phragmitis Rhizoma herbal tea. Methods A total of 181 Chinese patent medicine and formulations containing Phragmitis Rhizoma were retrieved from the Yaozh™ database. After screening, 97 formulations were included for data mining. The core composition of the herbal tea was identified through frequency statistics, cluster analysis, association rule analysis, and core complex network. The antipyretic effect and underlying mechanism were investigated using a dry yeast-induced febrile rat model. Results Data mining revealed that herbs used in Phragmitis Rhizoma-based formulations were predominantly sweet in taste and cold in property, primarily acting on the lung and stomach meridians. Eight traditional Chinese medicine combinations with high confidence and strong associations with Phragmitis Rhizoma were identified. Guided by traditional Chinese medicine theory, the core formulation was finalized as: Phragmitis Rhizoma, Gancao (Glycyrrhizae Radix et Rhizoma), Bohe (Menthae Haplocalycis Herba), Dazao (Jujubae Fructus), Maidong (Ophiopogonis Radix), Jiegeng (Platycodonis Radix), Jinyinhua (Lonicerae Japonicae Flos), and Korla fragrant pear. Animal studies showed that administration of low, medium, and high doses of the compound Phragmitis Rhizoma herbal tea significantly reduced the body temperature of febrile rats (P < 0.01). All dose groups significantly decreased serum tumor necrosis factor-α (TNF-α) levels, as well as hypothalamic prostaglandin E₂(PGE₂) and cyclic adenosine monophosphate (cAMP) levels (P < 0.01). Serum levels of interleukin‑6 (IL-6) were significantly reduced in the all groups (P < 0.05, 0.01). Conclusion The compound Phragmitis Rhizoma herbal tea can significantly reduce the body temperature of febrile rats. Its antipyretic mechanism is likely associated with decreasing serum levels of pyrogenic factors IL-6 and TNF-α, as well as reducing hypothalamus levels of pyrogenic, pro‑inflammatory, and algesic factors PGE₂ and cAMP content, there by causing a downward shift of the thermoregulatory set point.

R285

自治区重点研发计划项目“水生植物精深加工关键技术研发”（2023B02037-3-3）