目的 研究黄芪Astragalus membranaceus茎叶中降环阿尔廷烷型三萜类化学成分。方法 综合运用硅胶、ODS等柱色谱及高效液相色谱技术进行分离纯化，并通过现代波谱学方法鉴定化合物结构。采用MTT法评价化合物对PC12细胞的细胞毒性。结果 从黄芪茎叶70%乙醇提取物的正丁醇萃取部位中分离得到了2个降环阿尔廷烷型三萜，分别为1α,3β,12β,16β-tetrahydroxy-25,26,27-trinorcycloartan-24-oic acid methyl ester（1）和1α,3β,12β-trihydroxy-25,26,27-trinorcycloartan-24,16β-olide（2）。MTT实验显示，在5～100 μmol/L浓度内，化合物1和2对PC12细胞展现出不同程度的细胞毒性。结论 化合物1和2为新的降环阿尔廷烷型三萜，命名为黄芪茎叶环阿尔廷烷三萜K（1）和黄芪茎叶环阿尔廷烷三萜L（2）。化合物1和2对PC12细胞具有细胞毒性。

Objective To investigate trinorcycloartane triterpenoids from the stems and leaves of Astragalus membranaceus (AMSL). Methods Comprehensive separation and purification were performed using silica gel, ODS column chromatography, and high-performance liquid chromatography (HPLC). The structures of the compounds were identified by modern spectroscopic techniques. The cytotoxic activities of the compounds against PC12 cells were assessed using the MTT assay. Results Two trinorcycloartane triterpenoids were isolated from the n-butanol fraction of the 70% ethanol extract of AMSL, namely 1α,3β,12β,16β-tetrahydroxy-25,26,27-trinorcycloartan-24-oic acid methyl ester (1) and 1α,3β,12β-trihydroxy-25,26,27- trinorcycloartan-24,16β-olide (2). The MTT assay showed that compounds 1 and 2 exhibited varying degrees of cytotoxicity against PC12 cells within the concentration range of 5—100 μmol/L. Conclusions Compounds1 and 2 are identified as new trinorcycloartane triterpenoids, named cyclastraine K (1) and cyclastraine L (2), respectively. Both compounds 1 and 2 demonstrate cytotoxicity against PC12 cells.

R284.1

山西中医药大学杏林英才计划（2025XK17）;2025年度中医药产业发展现代化支撑项目（2025zyycy09）;山西省中医药管理局中医药创新团队建设计划（zyytd2024033）;山西中医药大学科技创新能力培育计划项目（2021PY-JC-09）;山西中医药大学学科建设项目（2025XK35）