目的 研究大叶山楝Aphanamixis polystachya枝叶的化学成分及其抗炎活性。方法 采用硅胶、C₁₈反相硅胶、Sephadex LH-20凝胶及MCI等多种柱色谱技术进行分离纯化，根据理化性质结合波谱数据鉴定化合物结构。采用脂多糖（lipopolysaccharide，LPS）诱导的RAW 264.7巨噬细胞炎症模型，对化合物进行抗炎活性评价。结果 从大叶山楝枝叶中分离得到11个二萜类化合物，分别鉴定为 (4S)-4-[(E)-11-(15,15-dimethyl-14-oxo-14,15-dihydrofuran-12-yl)-11,18-dihydroxy-7- methyloct-6-en-5-yl]-3-methyl-1(4H)-furanone（1）、nemoralisin B（2）、aphapolin A（3）、nemoralisin G（4）、nemoralisin J（5）、nemoralisin I（6）、nemoralisin H（7）、nemoralisin D（8）、nemoralisin A（9）、aphanamene B（10）和反式植醇（11）。化合物11对LPS诱导的RAW 264. 7细胞内NO的生成有微弱抑制作用，其半数抑制浓度（median inhibition concentration，IC₅₀）值为428.7 μmol/L。结论 化合物1为新化合物，命名为陵水暗罗素O（nemoralisin O）；化合物3、8和11为首次从大叶山楝中分离得到；化合物11显示出微弱的抗炎活性。

Objective To study the chemical constituents from the stems and leaves of Aphanamixis polystachya and their anti-inflammatory activities. Methods The compounds were isolated and purified by various column chromatography techniques, such as silica gel, C₁₈ reversed-phase silica gel, Sephadex LH-20 and MIC, and their structures were identified by physiochemical properties and spectroscopic data. The LPS-induced RAW 264.7 macrophage inflammation model was used to evaluate the anti-inflammatory activity. Results Eleven diterpenoids were isolated, which were elucidated as nemoralisin O (1), nemoralisin B (2), aphapolin A (3), nemoralisin G (4), nemoralisin J (5), nemoralisin I (6), nemoralisin H (7), nemoralisin D (8), nemoralisin A (9), aphanamene B (10), and trans-phytol (11). Compound 11 exhibited weak inhibitory activity against LPS-induced NO production with a half-maximal inhibitory concentration (IC₅₀) value of 428.7 μmol/L. Conclusion Compound 1 is identified as a new compound and named nemoralisin O (1). Compounds 3, 8, and 11 were isolated from this plant for the first time. Compound 11 showed weak anti-inflammatory activity.

R284.1

云南省科技重大专项（202002AA100007）