目的 研究灯盏细辛Erigeron breviscapus的化学成分及其体外抗胶质母细胞瘤和结肠癌活性。方法 采用大孔树脂柱色谱、中压制备色谱、高压制备色谱等多种方法进行分离和纯化，利用1D-NMR、2D-NMR、HR-ESI-MS、UV、IR等波谱技术对分离得到的化合物进行结构鉴定，采用CCK-8法对新化合物的活性进行筛选。结果 从灯盏细辛80%甲醇提取物中分离得到4个化合物，分别鉴定为反式-4-咖啡酰氧基-3-羟基-6-咖啡酰氧甲基-7,8-二氧杂环[3.2.1]辛烷-1-羧酸（1）、异绿原酸A（2）、绿原酸（3）和新绿原酸（4）；其中化合物1对人胶质瘤U87细胞和人结肠癌HT29细胞的半数抑制浓度（half inhibitory concentration，IC₅₀）分别为（13.31±0.77）、（14.83±0.92）μmol/L。结论 化合物1为新化合物，命名为新飞蓬酯乙；化合物1对肿瘤细胞的增殖具有一定的抑制作用。

Objective To investigate the chemical constituents of Dengzhanxixin [Erigeron breviscapus (Vant.) Hand.-Mazz.] and its in vitro anti-glioblastoma and colon cancer activities. Methods Various methods including macroporous resin column chromatography, medium-pressure preparative chromatography, and high-pressure preparative chromatography were employed to isolate and purify the 80% methanol extract of E. breviscapus. The compounds obtained were structurally identified using 1D-NMR, 2D-NMR, HR-ESI-MS, UV, IR, and other spectroscopic techniques. The activity of the new compounds was screened using the CCK-8 method. Results Four compounds were isolated from E. breviscapus and identified as trans-4-caffeoyloxy-3-hydroxy-6-caffeoyloxymethyl-7,8-dioxabicyclo[3.2.1]octane-1-carboxylic acid (1), isochlorogenic acid A (2), chlorogenic acid (3), and neochlorogenic acid (4), respectively. The half inhibitory concentration (IC₅₀) of compound 1 on human glioma U87 cells and human colon cancer HT29 cells was (13.31±0.77), (14.83±0.92) μmol/L, respectively. Conclusion Compound 1 is a new compound named neoerigeroster B; Compound 1 exhibits certain inhibitory effects on the proliferation of tumor cells.

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国家自然科学基金青年科学基金项目（82374076）;国家自然科学基金青年科学基金项目（81703744）;北京市自然科学基金青年项目（7244493）;北京市科学技术协会2023-2025年度青年人才托举工程项目（BYESS2023354）;中国中医科学院中药研究所国家自然科学基金培育专项（ZXKT23003）;北京中医药大学实验技术（操作规程）标准化研究项目（2023-syjs-01）