目的 建立木香Aucklandia lappa的高效液相色谱（HPLC）指纹图谱并对特征峰进行液相色谱串联三重四级杆质谱仪（LC-MS）分析，考察木香抗氧化、抗辐射的谱效关系。方法 对12批不同产地的木香药材进行HPLC分析，建立指纹图谱。采用LC-MS技术对指纹图谱中的共有特征峰进行结构分析。以1,1-二苯基-2-三硝基苯肼（DPPH）自由基清除率为抗氧化作用的药效指标，研究其抗氧化作用。采用MTT法检测辐射后的UC-MSC细胞活性，以细胞活力值为抗辐射作用的药效指标，研究其抗辐射作用。采用灰色关联分析法建立谱效关系，研究木香抗氧化与抗辐射的物质基础。结果 通过指纹图谱的建立，共标定了12个共有峰，其中12批药材相似度均大于0.971。通过文献比对和LC-MS数据解析，确定了3,5-O-二咖啡酰基奎宁酸、东莨菪内酯、木香烃内脂和去氢木香内酯等12个特征峰成分。通过清除DPPH自由基结果发现，12批木香均有抗氧化能力，谱效分析结果显示，去氢木香内酯同分异构体和去氢木香内酯与抗氧化能力有密切关系。通过细胞活力值显示，12批木香部分批次具有抗辐射能力。根据谱效关系分析，发现木香抗辐射与诱导细胞毒性的物质基础可能均是异木香酸甲酯、木香烃内脂和去氢木香内酯。结论 为木香药材的质量控制及研究其抗氧化和抗辐射作用，提供了一种有效、快速地评价方法。

Objective To establish HPLC fingerprint of Aucklandiae Radix, identify the compounds of common peaks and study the relationship between effects of antioxidant and radioresistance and spectrum of Aucklandiae Radix. Methods Twelve batches of Aucklandiae Radix from different areas were analyzed by HPLC and the fingerprint was established. LC-MS technique was used to analyze the structures of common peaks from the fingerprint. The scavenging rate of DPPH was taken as pharmacodynamics indicator of antioxidant effect and MTT method was used to detect the cell viability of UC-MSC cell as pharmacodynamics indicator of radioresistance effect. Grey correlation analysis was used to establish the spectrum-effect relationship and figure out the material basis of antioxidant and radioresistance of Aucklandiae Radix. Results Through the establishment of fingerprint, 12 common peaks were picked and the similarity of each sample was higher than 0.971. Through literature comparison and LC-MS data analysis, 12 common peak components such as 3,5-O-dicoffeacylquinic acid, scopoletin, costunolide and dehydrocostus were identified. All of 12 batches had antioxidant ability by the results of scavenging DPPH. According to spectrum-effect relationship, the isomer of dehydrocostus and the dehydrocostus were related to antioxidant ability. By the results of cell viability, some batches had the radioresistance ability. According to spectrum-effect relationship, it was found that the substance of radioresistance ability and induced cytotoxicity might be methyl isocostate, costunolide and dehydrocostus. Conclusion The proposed method offered an effective and fast way for quality control and estimating the effects of antioxidant and radioresistance of Aucklandiae Radix.

R286.2

中国医学科学院医学与健康科技创新工程国家医学健康科技战略平台与体系建设专项项目资助（2022-I2M-2-003）