目的 基于转录组数据筛选鉴定分析甘松Nardostachys jatamansi MADS-box转录因子家族。方法 利用生物信息学的方法全面分析甘松MADS-box基因家族成员的蛋白理化性质、亚细胞定位、基因结构、导肽、信号肽及跨膜结构域、系统进化树。结果 在甘松转录组数据中共鉴定出20个MADS-box转录因子，含有90～365个氨基酸，相对分子质量在10 179.96～41 707.68，理论等电点范围为4.87～10.43，主要在细胞核表达，均含有MADS保守结构域，均不含信号肽、导肽以及跨膜结构域。系统发育分析表明甘松MADS蛋白主要属于TypeII型。结论 利用生物信息学分析手段，鉴定了甘松MADS-box家族转录因子，为甘松深入研究甘松MADS-box蛋白的生物学功能提供参考，为甘松的药用成分合成调控机制提供研究基础，为培育优质甘松新品种提供依据。

Objective To identify and analyze MADS-box transcription factor family in Gansong (Nardostachys jatamansi DC.) based on transcriptome data. Methods The protein physicochemical properties, subcellular localization, gene structure, conducting peptide, signal peptide, transmembrane domain and phylogenetic tree of MADS-box gene family members in N. jatamansi were comprehensively analyzed by bioinformatics methods. Results A total of 20 MADS-box transcription factors were identified in the transcriptome data of N. jatamansi. And they have 90—365 amino acids, and the molecular weight ranged from 10 179.96 to 41 707.68. The theoretical isoelectric point (pI) ranged from 4.87 to 10.43. And most of MADS-box protein located in nucleus. All of them contained MADS conserved domains, none of which contains a signal peptide, a guide peptide or a transmembrane domain. The results of phylogenetic analysis showed that the MADS protein mainly in N. jatamansi belonged to Type II. Conclusion In this study, the MADS-box gene family members were identified by bioinformatics analysis, which provides a reliable reference for further study on the biological function of MADS-box protein in N. jatamansi, offers a research basis for the regulation mechanism of medicinal composition synthesis of N. jatamansi, which further provides a basis for cultivating new varieties of N. jatamansi with high quality.

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国家重点研发计划（2018YFC1706101）；四川省自然科学基金项目（2022NSFSC1588）；2021年国家民委领军人才支持计划，西南民族大学研究生创新型科研项目（CX2021SZ94）