目的 研究斜叶黄檀Dalbergia pinnata茎部化学成分，并初步考察其抗炎活性。方法 采用正相硅胶、Sephadex LH-20凝胶、ODS及制备型高效液相等柱色谱方法进行分离纯化，通过核磁共振波谱、质谱和红外光谱等技术进行结构鉴定；采用脂多糖（lipopolysaccharide，LPS）诱导RAW 264.7细胞一氧化氮生成模型对分离鉴定的化合物进行抗炎活性筛选。结果 从斜叶黄檀茎的甲醇提取物中分离得到4个肉桂酚类化合物，分别鉴定为黄檀亭F（1）、2-羟基异尖叶军刀豆苏合香烯（2）、异尖叶军刀豆苏合香烯（3）和黄檀亭C（4）。结论 化合物1为新化合物，化合物2～4为首次从斜叶黄檀中分离得到。化合物1对LPS诱导RAW 264.7细胞释放一氧化氮显示出抑制活性，半数抑制浓度为23.6 μmol/L。

Objective To study the chemical constituents and their anti-inflammatory activities from the stems of Dalbergia pinnata. Methods The constituents were separated and purified by various column chromatographic technologies (silica gel, Sephadex LH-20, ODS column chromatography) as well as HPLC. The structures were elucidated by MNR, MS and IR data. All compounds were evaluated for their inhibitory activity against LPS-activated NO production in RAW 264.7 cell model. Results Four compounds were isolated and identified as dalberatin F (1), 2-hydroxyisomucronustyrene (2), isomucronustylene (3) and dalberatin C (4). Conclusion Compound 1 is identified as a new cinnamylphenol, and compounds 2 —4 are isolated from this plant for the first time. Compound 1 exhibits inhibitory effect on NO production in LPS-induced RAW 264.7 cells, with the IC₅₀ value of 23.6 μ mol/L.

R284.1

广州中医药大学“双一流”与高水平大学学科协同创新团队重点项目（2021xk31）；广东省新药设计与评价重点实验室开放课题基金（2017B030314030）