目的 对绞股蓝Gynostemma pentaphyllum的总皂苷提取物进行化学成分研究。方法 综合应用多种色谱技术进行分离纯化，通过各种现代谱学技术对化合物进行结构鉴定。结果 从绞股蓝的总皂苷提取物中分离得到2个葫芦烷型三萜皂苷类化合物，分别鉴定为葫芦素L-2-O-{[a-L-吡喃鼠李糖基-（1→2）] [a-L-吡喃阿拉伯糖基-（1→3）]-β-D-吡喃葡萄糖基}-25-O-β-D-吡喃木糖苷（1）、葫芦素L-2-O-[a-L-吡喃鼠李糖基-（1→2）] [a-L-吡喃阿拉伯糖基-（1→3）]-β-D-吡喃葡萄糖苷（2）。结论 化合物1为1个新化合物，命名为绞股蓝葫芦皂苷A。

Objective To further study the chemical constituents from the total saponin extract of Jiaogulan (Gynostemma pentaphyllum). Methods The chemical constituents of G. pentaphyllum were separated and purified by chromatographic methods and structurally elucidated by means of various spectroscopic techniques. Results Two cucurbitane-type saponins were isolated from the total saponin extract of G. pentaphyllum, and identified as cucurbitacin L-2-O-{[a-L-rhamnopyranosyl-(1→2)] [a-L-arabinopyranosyl-(1→3)]-β-D-glucopyranosyl}-25-O-β-D-xylopyranoside (1), cucurbitacin L-2-O-[a-L-rhamnopyranosyl- (1→2)] [a-L-arabinopyranosyl-(1→3)]-β-D-glucopyranoside (2). Conclusion Compound 1 is a new compound named cucurbitane-gypenoside A.

R284.1

江苏省高校青蓝工程资助项目；大学生创新创业训练计划项目成果（202010316044S）；2020年江苏省研究生科研创新计划（KYCX20_0676）