目的 建立拟草果Amomum paratsao-ko药材的质量评价方法。方法 以鼠李柠檬素为参照峰确定共有峰，建立拟草果的HPLC指纹图谱；以鼠李柠檬素为内标，确定鼠李素、3，5-二羟基-7，4'-二甲氧基黄酮的相对校正因子并计算含量，实现一测多评。同时采用外标法测定3种成分的含量，比较计算值和实测值的差异。结果 建立了拟草果的HPLC指纹图谱，10批样品相似度均在0.9以上；以鼠李柠檬素为内标建立的鼠李素、3，5-二羟基-7，4'-二甲氧基黄酮的相对校正因子分别为1.300、0.885，重现性良好，10批拟草果样品一测多评法计算值与实测值无显著差异。结论 建立的HPLC指纹图谱和一测多评法可用于拟草果的质量评价。

Objective To establish a method for quality evaluation of Amomum paratsao-ko. Methods Using rhamonotin as the reference peak, the common peaks were determined to establish HPLC fingerprint of A. paratsao-ko. Rhamonotin was selected as internal reference and the relative correction factors (RCF) of three components were calculated and the contents were calculated by QAMS. Meanwhile, the contents of three components in A. paratsao-ko were determined by external standard method (EMS). The accuracy and feasibility of QAMS was evaluated by comparing the results between the measured values by EMS and calculated values by QAMS. Results The HPLC fingerprint of A. paratsao-ko was established and the similarity of fingerprints of 10 batches of test samples were above 0.9. The RCF of rhamnetin and 3,5-dihydroxy-7,4'-dimethoxy were 1.300, 0.885 and showed good reproducibility. The contents of three components showed no significant difference in the 10 batches of A. paratsao-ko determined by external standard method and QAMS. Conclusion The HPLC fingerprint and QAMS methods can be used to evaluate the quality of A. paratsao-ko.

R286.2

广西自然科学基金项目（2017GXNSFBA198091）