目的 建立半夏泻心汤水煎液的HPLC指纹图谱，研究处方中黄芩、黄连药材-饮片-全方水煎液的量值传递关系。方法 以《伤寒论》中记载的煎煮方法制备半夏泻心汤水煎液，建立半夏泻心汤HPLC指纹图谱，对特征峰进行归属；以转移率、出膏率、指纹图谱的相似度为指标，对处方中黄芩、黄连药材-饮片-全方水煎液的量值传递关系进行分析。结果 黄芩中指标性成分黄芩苷从药材到饮片的转移率为81.08%~119.82%；饮片到全方水煎液的黄芩苷的转移率为44.55%~59.05%，黄连中指标性成分小檗碱从药材到饮片的转移为66.65%~97.51%；饮片到全方水煎液的小檗碱的转移率为7.83%~22.93%；全方的出膏率为12.99%~17.21%；15批全方指纹图谱的相似度为0.978~0.999。结论 该指纹图谱所建立的质量评价方法适用于黄芩、黄连药材-饮片-全方水煎液质量控制，可为经典名方等质量评价办法的制定提出一定的参考。

Objective HPLC fingerprints of Banxia Xiexin Decoction (BXD) was established to analyze the quality value transmitting relationship between medical herbs-decoction pieces-whole decoction of Scutellariae Radix (SR) and Coptidis Rhizoma (CR). Methods BXD was prepared by the decoction method recorded in Treatise on Febrile Diseases. BXD HPLC fingerprints was established, and the characteristic peaks were analyzed; The transfer rate, the dry extract rate and the similarity of fingerprint were used as indicators to analyze the quality value transmitting relationship between medical herbs-decoction pieces-whole decoction of SR and CR. Results The transfer rate of baicalin from medical herbs to decoction pieces was between 81.08% and 119.82%; The transfer rate of baicalin from decoction pieces to BXD was between 44.55% and 59.05%; The transfer rate of berberine from medical herbs to decoction pieces was between 66.65% and 97.51%; The transfer rate of berberine from decoction pieces to BXD was between 7.83% and 22.93%; And the dry extract rate of the whole decoction was between 12.99% and 17.21%. The similarity of fingerprints of 15 batches of BXD ranged from 0.978 to 0.999. Conclusion The quality evaluation method established by the fingerprint is applicable to the quality control of medical herbs-decoction pieces-whole decoction of SR and CR, which provides some reference for the establishment of the quality evaluation method of famous classic formula.

R286.02

中央高校基本科研业务费专项资金资助（2020-JYB-ZDGG-031）