目的 研究续断"发汗"前后水煎液HPLC指纹图谱与促进大鼠成骨细胞、人成骨样MG-63细胞增殖药效的谱效关系，寻找续断发汗前后发挥药效的物质基础，为明确"发汗"对药效的影响提供依据。方法 采用DAD检测器建立续断"发汗"前后水煎液的HPLC指纹图谱，采用灰色关联度分析法建立其谱效关系。结果 14和4号峰代表的化学成分与成骨细胞的增殖分化及MG-63细胞的增殖都具有较高的关联度，关联度均在0.7以上。与成骨细胞增殖、MG-63细胞增殖、碱性磷酸酶（ALP）活性3个药效指标关联度排序比较靠前的特征峰有14、4、6、16、13、11、5号峰。结合前期研究推断，这些峰可能为川续断皂苷VI、绿原酸、马钱苷、川续断皂苷IV同分异构体、川续断皂苷X、异绿原酸C、咖啡酸。结论 川续断皂苷VI、绿原酸、马钱苷、川续断皂苷IV同分异构体、川续断皂苷X、异绿原酸C、咖啡酸可能是续断"发汗"前后影响细胞增殖分化作用的主要物质基础，进而影响其药效。

Objective To study the spectrum-effect relationships of fingerprints of sweated and crude Dipsaci Radix on cell proliferation and differentiation, and find out the material basis of efficacy before and after sweating in order to provide the basis for the impact of the efficacy. Methods The DAD detector was used to establish HPLC fingerprints of sweated and crude Dipsaci Radix, and the relationship between the spectrum and efficiency was established by gray relational analysis. Results The chemical composition of peaks 14 and 4 were highly correlated with the proliferation and differentiation of osteoblasts and the proliferation of MG-63 cells. The correlations were all above 0.7. The three pharmacophore indicators associated much with the characteristic peaks 14, 4, 6, 16, 13, 11, and 5. Combined with the previous analysis, these peaks represented respectively asperosaponin VI, chlorogenic acid, loganin, asperosaponin IV isomers, dipsacoside X, chlorogenic acid C, and caffeic acid. Conclusion Asperosaponin VI, chlorogenic acid, loganin, asperosaponin IV isomers, dipsacoside X, chlorogenic acid C, and caffeic acid may be the main material basis for the effect of cell proliferation and differentiation, thus affecting its efficacy.

国家自然科学基金青年科学基金资助项目（81303224）；浙江中医药大学科研基金项目-2018国家自然科学基金预研项目（2018ZG34）；中华中医药学会青年人才托举工程项目（QNRC2-C12）；浙江省药品监督管理局委托的中药材产地加工项目