目的 建立高效液相色谱-串联质谱（HPLC-MS/MS）方法，研究芪苈强心胶囊中黄芪甲苷、毛蕊异黄酮葡萄糖苷和芒柄花素在大鼠体内的药动学特征。方法 SD大鼠ig给予芪苈强心胶囊混悬液1.3 g/kg，于不同时间点经目内眦静脉丛取血，采用HPLC-MS/MS法测定黄芪甲苷、毛蕊异黄酮葡萄糖苷和芒柄花素的血药浓度。色谱柱为Agilent Zobrax XDB-C₁₈（50 mm×4.6 mm，3.5 μm）；流动相为0.1%甲酸水溶液（A）-0.1%甲酸甲醇溶液（B），梯度洗脱；扫描方式为多反应监测（MRM）正离子模式检测。通过DAS 3.0软件拟合计算药动学参数。结果 大鼠ig芪苈强心胶囊后，血浆中黄芪甲苷、毛蕊异黄酮葡萄糖苷和芒柄花素的药动学参数达峰时间（t_max）分别为（0.67±0.00）、（0.67±0.06）、（0.17±0.00）h，峰浓度（C_max）分别为（120.46±45.03）、（31.49±12.00）、（5.17±1.13）ng/mL，药时曲线下面积（AUC_0~t）分别为（320.92±79.10）、（103.26±47.63）、（14.70±5.48）h·ng/mL，半衰期（t_1/2）分别为（5.74±0.78）、（6.05±3.34）、（5.07±2.13）h。结论 该检测方法专属性强、重复性好，适用于芪苈强心胶囊中黄芪甲苷、毛蕊异黄酮葡萄糖苷和芒柄花素在大鼠体内的药动学研究。

Objective A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to study the pharmacokinetic behaviors of astragaloside, calycosin-7-glucoside, and formononetin in rat plasma after oral administration of Qili Qiangxin Capsule. Methods SD rats were given 1.3 g/kg Qili Qiangxin Capsule suspension by gavage. Blood samples were collected from inner canthus at different time points. The concentration of the components was determined by HPLC-MS/MS. The chromatographic column was Agilent Zobrax XDB-C₁₈ (50 mm×4.6 mm, 3.5 μm); The mobile phase was 0.1% formic acid-water (A) and methanol solution containing 0.1% formic acid (B), gradient elution; And the scanning mode was multi-reflection monitoring (MRM) with positive ion mode. The pharmacokinetic parameters were calculated by DAS 3.0 software fitting. Results The pharmacokinetic parameters t_max of astragaloside, calycosin-7-glucoside and formononetin in rat plasma were (0.67 ±0.00), (0.67 ±0.06), and (0.17 ±0.00) h, C_max were (120.46 ±45.03), (31.49 ±12.00), and (5.17 ±1.13) ng/mL, AUC_0-t were (320.92 ±79.10), (103.26 ±47.63), and (14.70 ±5.48) h·ng/mL, t_1/2 were (5.74 ±0.78), (6.05 ±3.34), and (5.07 ±2.13) h, respectively. Conclusion The method is proved to be specific and repeatable, which is suitable for determination of astragaloside, calycosin-7-glucoside, and formononetin in rat plasma for pharmacokinetic study.

国家自然科学基金资助项目（81774227）；天津市自然科学基金资助项目（17JCZDJC34600）；天津市卫生和计划生育委员会中医、中西医结合科研课题（2015050）