目的 优选川芎产地加工与饮片炮制一体化工艺（简称一体化），为川芎产地加工与饮片炮制一体化工艺提供依据。方法 采用单因素和正交试验法对鲜川芎含水量、切片厚度和烘干温度这3个显著影响因素进行考察，以川芎饮片外观性状和有效成分含量（浸出物、阿魏酸和挥发油）为考察指标，通过综合加权评分法对一体化工艺进行优选研究；按照优选出的最佳工艺加工3批一体化饮片，考察工艺的稳定性和重复性；同时按照《中国药典》2015年版规定方法加工3批对应传统饮片，比较2种饮片质量；采用硝酸甘油复制偏头痛大鼠模型，通过记录大鼠甩头次数、挠头次数和测定血清中NO水平、一氧化氮合酶（NOS）活性4个指标来比较2种不同加工方式饮片治疗大鼠偏头痛的效果。结果 最佳一体化工艺为鲜川芎晾晒至含水量为28%，切2 mm厚片，置干燥箱内50℃烘干（约6 h）；一体化工艺具有良好的稳定性和重复性，2种工艺饮片质量接近；与模型组比较，2种工艺川芎饮片均能显著减少大鼠甩头和挠头次数（P<0.01、0.001），降低NOS活性 （P<0.01），一体化饮片能显著降低血清NO水平（P<0.01），2种工艺饮片药效学作用相似。结论 川芎一体化饮片与传统饮片在性状、有效成分含量以及治疗偏头痛方面具有良好的相似性，而且一体化工艺从源头规范了川芎饮片生产，在确保饮片质量的前提下缩短生产周期，节约生产成本，是一种值得推广的中药饮片生产模式。

Objective To optimize the integration technology on field processing and processing crude drugs of Ligusticum chuanxiong (LC) and provide the scientific evidence for the integration of LC. Methods Single factor test and orthogonal method was used to investigate the three significant influencing factors, which were water content, drying temperature, and slice thickness. Based on the appearance character and active component (ferulic acid, volatile oil, and extract) of LC, the integrated process was selected by comprehensive scoring method. To investigate the stability of the process, three batches of integrated slices were processed according to the optimized integrated process; And three batches of corresponding traditional slices were processed according to the method stipulated in Chinese Pharmacopoeia (2015 edition), and the quality of the two kinds of slices was compared. The rat migraine model was induced by nitroglycerin. The effects of two different processed slices on migraine were compared by recording the times of head flick and head scratching, the level of NO in serum and the activity of NOS in serum of rats. Results The optimum integrated process was as follows:drying fresh LC until water content was 28%, cutting 2 mm thick slices, drying at 50℃ in drying box (about 6 h). The integrated process had good stability and repeatability, and the quality of integrated slices was close to that of traditional slices. Compared with the model group, two processed slices could significantly reduce the number of head flick and head scratch (P < 0.01, 0.001), and decreased the activity of NOS (P < 0.01), and the level of NO decreased significantly (P < 0.01). The pharmacodynamics of the two process pieces was similar. Conclusion The integrated processing and traditional processing of Chuanxiong Rhizoma decoction has good similarity in character, effective component content and treatment of migraine. Moreover, the integrated processing has standardized the production of Chuanxiong Rhizoma decoction slices from the source. Under the premise of ensuring the quality of Chinese herbal slices, the production cycle is shortened and the production cost is saved. It is a kind of traditional Chinese medicine slices production mode which is worth popularizing.

国家自然基金（国家基础科学人才培养基金）（J1310034-07）；四川省科技厅应用基础计划课题（2016JY0076）；四川省科技厅应用基础计划课题（2016JY019）；四川省科技厅扶贫资助项目（2018NFP0112）