目的 研究玫瑰Rosa rugosa花蕾的化学成分。方法 运用硅胶、凝胶、MCI-gel树脂及PR-HPLC等多种色谱技术对玫瑰花蕾的化学成分进行分离纯化, 并根据理化性质和波谱数据鉴定化合物的结构。结果 从玫瑰花蕾中分离得到了3个异黄酮类化合物, 分别鉴定为6,8-二羟基-4',7-二甲氧基异黄酮(1)、樱黄素(2)、红车轴草素(3)。细胞毒活性筛选显示化合物1对A549和PC3细胞的IC₅₀值分别为2.6和3.2 μmol/L。结论 化合物1为新化合物, 命名为玫瑰异黄酮, 化合物2和3首次从玫瑰花蕾中分离得到;化合物1表现出较强的细胞毒活性。

Objective To study the chemical constituents from the flower buds of Rosa rugosa. Methods The chemical constituents from the flower buds of R. rugosa were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatography and high performance liquid chromatography (HPLC) methods. Their structures were elucidated by spectroscopic methods and physicochemical properties. Results Three isoflavones, 6,8-dihydroxy-4',7-dimethoxyisoflavone (1), prunetin (2), and pratensein (3) were isolated from the flower buds of R. rugosa. Conclusion Compound 1 is a new compound named rosa isoflavone. Compounds 2 and 3 are isolated from R. rugosa for the first time. Compound 1 displays the stronger cytotoxicity against A549 and PC3 cells with IC₅₀ values of 2.6 and 3.2 μmol/L, respectively.

国家自然科学基金资助项目(21032085, 31460144);云南省高校科技创新团队(IRTSTYN 2014-11);云南民族大学研究生创新项目(2014YJY86)