目的 用代谢物组学方法研究钩藤多动合剂(简称多动合剂)的药效作用及其治疗儿童多动症的生化机制,观察药物对小鼠脑内神经递质的影响。方法 采用攀爬实验和自发行为实验研究多动合剂对去水吗啡小鼠模型的药效作用,同时用高效液相色谱-电化学检测方法研究其生化机制,观察药物对小鼠脑内及血浆中单胺类神经递质多巴胺(DA)和去甲肾上腺素(NE)的影响。结果 去水吗啡激动黑质纹状体系统内DA受体,使系统内DA功能增强,引起小鼠攀爬行为和自发活动增强,多动合剂可抑制去水吗啡引起的小鼠的攀爬行为,并减弱自发活动的增强。对去水吗啡引起的血浆中DA和NE含量的改变及脑组织中DA含量的改变,多动合剂均能予以校正,使各项指标接近空白对照组水平。实验结果表明多动合剂可提高小鼠血浆中DA含量,并降低脑组织中DA的含量。结论 多动合剂能调整单胺类神经递质的失衡以此达到治疗多动综合征的目的。

Objective To investigate pharmacodynamics and its biochemical mechanism for treating hyperactivity syndrome in children with Uncaria—stem Mistura for Hyperactivity Syndrome(UMHS) whose effect on intracephalic neurotransmitter of mice was observed by metabonomic method．Methods The climbing test and spontaneous behavior test were used to study the behavior pharmacodynamie action of UMHS in the mouse model treated with apomorphine(APO)．The biochemical mechanism and the effect of UMHS on two kinds of monoamine neurotransmitters，dopamine(DA)and nor—epinephrine(NE)in mouse brain and plasma were observed by HPLC—ECD method．Results DA receptor in intercalatum striatum system stimulated by APO enhanced the DA function and potentiated the climbing and spontaneous behavior of mice．which could be inhibited and attenuated by UMHS．Both the changes of DA and NE in plasma induced by APO and DA in brain tissue could be adjusted by UMHS with making them close to the level in the control group．The Results showed that DA in plasma could be increased．while in brain decreased by UMHS．Conclusion AU these indicate that the therapeutic effect of UMHS on hyperactivity syndrome is associated with the biochemical base on which the unbalance of monoamine neurotransmitter has been regulated．