目的 对胡芦巴 Trigonella foenum-graecum种子所含的皂苷成分作深入研究。方法 采用硅胶 H柱色谱,干柱色谱等方法从总皂苷中分离纯化皂苷成分并通过 1 3C-NMR,FAB-MS,DEPT等波谱法及部分水解获得次生苷的方法测定了化学结构。结果 从总皂苷中分出两个新皂苷 B和 C,均为薯蓣皂苷元的四糖苷。经测定皂苷 B的化学结构为:薯蓣皂苷元-3-O-α-L-鼠李吡喃糖 (1→3)-α-L-鼠李吡喃糖 (1→4)-β-D-葡萄吡喃糖 (1→4)-β-D-葡萄吡喃糖苷;皂苷 C的结构为薯蓣皂苷元-3-O-β-D-葡萄吡喃吡喃糖 (1→4)-α-L-鼠李吡喃糖 (1→4)-β-D-葡萄吡喃糖(1→4)-β-D-葡萄吡喃糖苷。结论 皂苷 B和 C是两个新的四糖皂苷。

Object To do detail investigation of the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum graecum L) Methods The pure saponins from the total saponins were isolated by employing the column chromatography and dry column chromatography of silica gel H Their chemical structures were elucidated by 13 C NMR, FAB MS, DEPT spectroscopic evidence and the Results of the fraction hydrolysis of acquiring their secondary glucosides were obtained Results Two new saponins B and C were isolated and both were the glucosides consisted by four molecules of sugar with diosgenin The chemical structure of B is: diosgenin 3 O α L rhamnopyranosyl (1→3) α L rhamnopyranosyl (1→4) β D glucopyranosyl (1→4) β D glucopyranoside And saponin C is: diosgenin 3 O β D glucopyranosyl (1→4) α L rhamnopyranosyl (1→4) β D glucopyranosyl (1→4) β D glucopyranoside Conclusion Saponins B and C are two new ones with four molecules of sugar respectively.