目的 利用生物信息学的方法分析决明WRKY转录因子家族成员，为深入研究其生物学功能提供参考。方法 基于决明基因组注释信息，利用生物信息学的方法对WRKY家族成员的理化性质、进化关系、基因结构、保守基序进行分析。通过实时荧光定量PCR的方法（quantitative real-time PCR，qRT-PCR），测定茉莉酸甲酯（methyl jasmonate，MeJA）处理条件下决明WRKY与蒽醌类代谢物合成途径关键酶基因的表达模式。结果 从决明基因组中共鉴定出82个SoWRKYs家族成员，编码氨基酸数量为69～1 394 aa，蛋白相对分子质量为8 006.1～158 759.11，等电点4.68～10.13，除SoWRKY72为疏水性蛋白以外，其余SoWRKYs均为亲水性蛋白。大部分SoWRKY蛋白定位于细胞核。82个SoWRKYs不均匀地分布于13条染色体上。根据基因结构的特点将SoWRKYs家族成员分为3个亚家族。顺式作用元件分析表明SoWRKYs的表达受到光、激素和胁迫的影响。qRT-PCR分析的结果表明7个SoWRKYs对于MeJA均有响应，其中SoWRKY6和SoWRKY38在6 h表达水平最高，而SoWRKY10/39/72/73的表达峰值出现在12 h。SoWRKYs与蒽醌代谢途径关键酶基因之间的相关性分析表明，SoWRKYs能够调节蒽醌类物质合成途径SoCHS、SoICS、SoMenB等酶基因的表达。结论 为解析SoWRKYs对MeJA的响应提供了参考，也为深入探究SoWRKYs在蒽醌类物质积累调控中的功能奠定了理论基础。

Objective To identify the WRKY transcription factor family by bioinformatics, and to provide a reference for further research on their biological function. Methods Based on the annotation information of the Senna obtusifolia genome, bioinformatics methods were employed to analyze the physicochemical properties, evolutionary relationships, gene structures, and conserved motifs of WRKY family. Real-time fluorescence quantitative PCR (qRT-PCR) was used to determine the expression patterns of WRKY and key enzyme genes involved in anthraquinone synthesis under the condition of methyl jasmonate (MeJA) treatment. Results A total of 82 SoWRKYs were identified from the S. obtusifolia genome, with their encoded proteins ranging from 69 to 1 394 amino acids in length. The molecular weight of SoWRKYs ranged from 8 006.1 to 158 759.11 and the isoelectric points ranged from 4.68 to 10.13. Except for SoWRKY72, which is a hydrophobic protein, all other SoWRKYs were hydrophilic proteins. Most of SoWRKYs localized in nucleus. 82 SoWRKYs were unevenly distributed on 13 chromosomes. SoWRKYs were divided into three subfamilies based on the domain characters. Cis-acting element analysis indicated that SoWRKYs can response to light, hormones, and stress. The results of qRT-PCR analysis showed that seven SoWRKYs can respond to MeJA, among which SoWRKY6 and SoWRKY38 had the highest expression levels at 6 h, while the expression peak of SoWRKY10/39/72/73 appeared at 12 h. Correlation analysis between SoWRKYs and key enzyme genes showed that SoWRKYs could regulate the expression of key enzyme genes such as SoCHS, SoICS and SoMenB. Conclusion The present study provides a reference for in-depth analysis of the response mechanism of SoWRKYs to MeJA. In addition, this study also lays a theoretical foundation for the further exploration of SoWRKYs function in regulating anthraquinone accumulation.

R286.12

国家自然科学基金项目（32460096）；延安市高层次人才项目（2019-23）