目的 研究不同产地藤茶药材(显齿蛇葡萄Ampelopsis grossedentata茎叶)贮藏期间有效成分的含量变化,为藤茶药材的贮藏条件和有效期的制定提供依据。方法 采用加速试验和长期试验法,以藤茶药材的性状、水分、二氢杨梅素含量和HPLC-PDA指纹图谱为考察指标,采用主成分分析法和相关性分析法考察成分变化之间的相关性,并采用紫外法、HPLC-MS质谱及圆二色谱法对未知成分及转化机制进行解析。结果 在加速试验9个月过程中,藤茶中二氢杨梅素的含量基本稳定,水分有所增加,但仍符合标准要求;杨梅素含量呈升高趋势,未知峰呈下降趋势,二者变化具有显著的相关性,未知峰推测为二氢杨梅素的异构体。在36个月的长期试验过程中,藤茶水分、二氢杨梅素含量及各特征峰的单位质量峰面积均趋于稳定。结论 藤茶常温条件下,贮藏3年内质量稳定,其贮藏过程应避免高温高湿的环境。

Objective To study the changes in the content of active ingredients in vine tea (Ampelopsis grossedentata, using stems and leaves as medicinal parts) medicinal materials during storage from different origins, providing a basis for determining the storage conditions and shelf life of vine tea medicinal materials. Methods Accelerated testing and long-term testing methods were utilized, with the traits, moisture content, dihydromyricetin content, and HPLC-PDA fingerprints of vine tea, as well as the content of dihydromyricetin and HPLC-PDA fingerprint patterns as indicators. Principal component analysis and correlation analysis were employed to investigate the correlation between changes in components, and UV spectroscopy, HPLC-MS, and circular dichroism spectroscopy were used to analyze unknown components and conversion mechanisms. Results During the nine-month accelerated testing process, the content of dihydromyricetin in vine tea remained relatively stable, with a slight increase in moisture content, which still met standard requirements. The the area of unknown peaks showed an upward trend, there was a significant correlation between the two changes, and the unknown peaks were speculated to be isomers of dihydromyricetin. During the 36-month long-term testing process, moisture content, dihydromyricetin content, and the peak area per unit mass of each characteristic peak of vine tea all tended to stabilize. Conclusion Under normal temperature conditions, vine tea medicinal materials can maintain stable quality for up to three years. During the storage process, it is important to avoid environments with high temperature and humidity.

R286.2

湖北省技术创新计划重点研发专项（2023BCB063）；湖北省技术创新计划重点研发专项（2023BCB130）；湖北省技术创新计划重点研发专项（2024BCB034）