目的 建立不同产地17批茺蔚子Leonuri Fructus药材指纹图谱,对其进行成分研究并结合化学计量学分析进行质量评价。方法 通过超高效液相色谱法(UPLC),以YMC Trait C₁₈(150 mm×2.1 mm,1.9 μm)为色谱柱,乙腈-0.1%磷酸溶液为流动相,梯度洗脱,体积流量为0.3 mL/min,柱温为28 ℃,检测波长为205 nm,进样体积为2 μL。采用中药指纹图谱相似度评价软件进行相似度评价,运用化学计量学对不同产地茺蔚子进行分析,同时对益母草苷B、刺桐碱、7R,8S-7′,8′-二羟基-二氢脱氢松柏醇-9-O-β-D-葡萄糖苷、落叶松脂素-9-O-β-D-葡萄糖苷、环益母草肽A进行含量测定。结果 建立的指纹图谱共标识出11个共有峰,经与对照品比对,指认6个成分;经聚类分析和主成分分析(principal component analysis,PCA),可将不同茺蔚子聚为3类,并通过正交偏最小二乘判别法(orthogonal partial least squares-discriminant analysis,OPLS-DA)筛选出峰5、峰4、峰7(刺桐碱)、峰11(环益母草肽A)、峰10(落叶松脂素-9-O-β-D-葡萄糖苷)、峰6(益母草苷B)、峰9(7R,8S-7′,8′-二羟基-二氢脱氢松柏醇-9-O-β-D-葡萄糖苷)和峰8可以作为区分不同产地茺蔚子药材的主要差异成分。17批茺蔚子中益母草苷B、刺桐碱、7R,8S-7′,8′-二羟基-二氢脱氢松柏醇-9-O-β-D-葡萄糖苷、落叶松脂素-9-O-β-D-葡萄糖苷、环益母草肽A的含量范围分别为11.434～57.262、2.386～7.825、0.068～0.236、0.101～0.439、0.732～1.604 mg/g;经方法学考察,各成分呈现良好的线性关系。结论 建立的茺蔚子指纹图谱能反映不同产地的茺蔚子样品差异,多成分含量测定方法稳定、可靠,为茺蔚子质量评价提供参考。

Objective To establish the fingerprint spectra of 17 batches of Leonuri Fructus (Chongweizi) from different origins. The components were studied and the quality was evaluated in combination with chemometrics analysis. Methods By ultra-performance liquid chromatography (UPLC), YMC Trait C₁₈ (150 mm × 2.1 mm, 1.9 μm) was used as the chromatographic column, and acetonitrile -0.1% phosphoric acid solution was used as the mobile phase, gradient elution was performed. The flow rate was 0.30 mL/min, the column temperature was 28 ℃, the detection wavelength was 205 nm, and the injection volume was 2 μL. Traditional Chinese medicine (TCM) fingerprint similarity evaluation software was used for similarity evaluation, and chemometrics was applied to analyze Leonuri Fructus from different origins. Meanwhile, the contents of leonuriside B, lenticin, 7R,8S-7′,8′-dihydroxydihydrodehydroconiferyl alcohol 9-O-β-D-glucopyranoside, lariciresinol-9-O-β-D-glucopyranoside, cycloleonuripeptide A were determined by similarity analysis and chemometrics analysis. Results The established fingerprint spectra identified a total of 11 common peaks. Through the identification of reference substances combined with the preparation and separation technology, a total of six components were identified by comparison with reference substances. Through cluster analysis and principal component analysis, different Leonuri Fructus can be clustered into three categories, and through OPLS-DA, peaks 5, 4, 7 (lenticin), 11 (cycloleonuripeptide A), 10 (lariciresinol-9-O-β-D-glucopyranoside), 6 (leonuriside B), 9 (7R,8S-7′,8′-dihydroxydihydrodehydroconiferyl alcohol 9-O-β-D-glucopyranoside), 8 were screened out as the main differential components for distinguishing the medicinal materials of Leonuri Fructus from different origins.The contents ranged of leonuriside B, lenticin, 7R,8S-7′,8′-dihydroxydihydrodehydroconiferyl alcohol 9-O-β-D-glucopyranoside, Lariciresinol-9-O-β-D-glucopyranoside and cycloleonuripeptide A in the 17 batches of Leonuri Fructus were 11.434—57.262, 2.386—7.825, 0.068—0.236, 0.101—0.439, 0.732—1.604 mg/g. According to the methodology, each component showed a good linear relationship. Conclusion The fingerprint spectra of Leonuri Fructus established in this paper can reflect the differences of Leonuri Fructus samples from different origins, and established multi-component content determination method is stable and reliable, which can provide a reference for the quality evaluation of Leonuri Fructus.

R286.2

2022年佛山市南海区重点领域科技攻关专项（南科﹝2023﹞20号-18）