目的 研究涠洲岛来源的海洋真菌Penicillium sp. MDW-627产生的次级代谢产物及其促血管生成活性。方法 综合运用红外光谱(IR)、紫外吸收光谱(UV)、高分辨质谱(HR-ESI-MS)及核磁共振(1D/2D NMR)技术,鉴定化合物的结构,并通过圆二色谱(ECD)以及文献比对确定化合物的绝对构型。对化合物进行基于斑马鱼模型的促血管生成活性测试。结果 分离鉴定了1个新嗜氮酮类化合物以及9个已知化合物:5-Cl-peniazaphilone G(1)、sclerotioramine(2)、(＋)-sclerotiorin(3)、11-epi-geumsanol B(4)、isochromophilone VI(5)、peniazaphilone C(6)、弯孢菌霉素(7)、diaporthelactone(8)、8-hydroxy-6-methoxy-3-methylisocoumarin(9)、异麦芽酚(10)。促血管生成活性结果显示化合物5在浓度为40 μmol/L时具有促血管生成活性。结论 化合物1为新的嗜氮酮类化合物,命名为5-Cl-盘尼阿扎菲酮,化合物5具有促血管生成活性。

Objective To investigate the secondary metabolites produced by the marine-derived fungus Penicillium sp. MDW-627 isolated from Weizhou Island and evaluate their pro-angiogenic activity. Methods The structures of the compounds were elucidated using a combination of infrared spectroscopy (IR), ultraviolet-visible spectroscopy (UV), High-Resolution ElectroSpray Ionization Mass Spectrometry (HR-ESI-MS), and multidimensional nuclear magnetic resonance (1D/2D NMR). The absolute configuration of the compounds was determined by electronic circular dichroism (ECD) spectroscopy in combination with literature comparison. The pro-angiogenic activity of the compounds was assessed using a zebrafish model. Results A new azaphilone derivative and nine known compounds were isolated and identified: 5-Cl-peniazaphilone G (1), sclerotioramine (2), (+)-sclerotiorin (3), 11-epi-geumsanol B (4), isochromophilone VI (5), peniazaphilone C (6), curvularin (7), diaporthelactone (8), 8-Hydroxy-6-methoxy-3-methylisocoumarin (9), isomaltol (10). The pro-angiogenic activity assay revealed that compound 5 exhibited significant vascular growth-promoting effects at a concentration of 40 μmol/L. Conclusion Compound 1 was identified as a new azaphilone derivative, designated as 5-Cl-peniazaphilone, and compound 5 demonstrated significant pro-angiogenic activity.

R284.1

国家自然科学基金青年项目（82204276）；广西自然科学基金资助项目（2025GXNSFAA069557，2019GXNSFBA185002）；广西高等学校千名中青年骨干教师培育计划