目的 建立蕤仁Prinsepiae nux饮片多指标定量检测方法，结合化学模式识别及CRITIC（criteria importance though intercrieria correlation）-逼近理想解排序（technique for order preferenceby similarity to ideal solution，TOPSIS）法评价不同产地蕤仁饮片质量。方法 采用高效液色谱法，以槲皮素、β-谷甾醇为内参物，分别计算没食子酸、原儿茶酸、香草酸、山柰酚与槲皮素，熊果酸、齐墩果酸、委陵菜酸、胡萝卜苷与β-谷甾醇的相对校正因子，对蕤仁饮片中10个成分进行定量检测；同时检测醇溶性浸出物、总灰分和酸不溶性灰分含量。采用化学模式识别以及CRITIC-TOPSIS法评价不同产地蕤仁饮片质量。结果 外标法方法学验证结果良好；各相对校正因子重复性良好；外标法与一测多评（quantitative analysis of multi-components by single marker，QAMS）法计算结果无明显差异（P＞0.05）。主成分分析（principal component analysis，PCA）结果显示不同产地16批蕤仁饮片分成3类；正交偏最小二乘法-判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）结果显示没食子酸、槲皮素、β-谷甾醇、熊果酸、委陵菜酸、齐墩果酸和山柰酚变量重要性投影值均＞1，是区分不同产地蕤仁饮片质量的差异性标志物；CRITIC-TOPSIS分析结果显示，法16批蕤仁饮片综合质量评价指标的相对贴近度为0.407 2～0.593 5，其中陕西、甘肃和山西产蕤仁饮片排名靠前，样品质量较优。结论 通过建立蕤仁饮片中多指标定量，结合化学模式识别及CRITIC-TOPSIS分析评价了不同产地蕤仁饮片质量，为蕤仁饮片质量标准的研究及其质量标志物的研究提供参考。

Objective To compare the quality of Ruiren (Prinsepiae nux) from different producing areas by chemical pattern recognition method and CRITIC-TOPSIS method. Methods The quantitative analysis of 10 components in Prinsepiae nux was performed by HPLC with quercetin and β-sitosterol as the reference substances. The relative correction factors of gallic acid, protocatechuic acid, vanillic acid and kaempferol with quercetin, ursolic acid, oleanolic acid, tormentic acid and eleutheroside A with β- sitosterol were calculated respectively. At the same time, the contents of alcohol-soluble extract, total ash and acid-insoluble ash in Prinsepiae nux were detected. The quality of Prinsepiae nux from different producing areas was evaluated by chemical pattern recognition and CRITIC-approximate ideal solution sorting method. Results The results of methodological verification of external standard method were good. The repeatability of each relative correction factor was good. There was no significant difference by external standard method and QAMS method (P > 0.05). The results of principal component analysis (PCA) showed that 16 batches of samples could be divided into three categories. The results of orthogonal partial least squares discriminant analysis (OPLS-DA) showed that the VIP values of gallic acid, quercetin, β-sitosterol, ursolic acid, tormentic acid, oleanolic acid and kaempferol in 13 indexes were > 1, which were the differential marker to distinguish the quality of Prinsepiae nux from different producing areas. The results of CRITIC-TOPSIS analysis showed that the relative closeness of the comprehensive quality evaluation index of 16 batches of Prinsepia uniflora was 0.4072—0.5935, among them, Prinsepiae nux from Shanxi, Gansu and Shanxi ranked top, and the sample quality was better. Conclusion The quality of Prinsepiae nux was evaluated by establishing a multi-index quantitative model and combining chemical pattern recognition and CRITIC-TOIS analysis, which can provide a reference for the study of the quality standard of Prinsepiae nux and its potential quality markers.

R286.2

河南省高等学校重点科研指导计划项目（20B550007）