目的 研究箭叶淫羊藿Epimedium sagittatum正丁醇部位黄酮类化学成分及其潜在抗肺纤维化活性。方法 运用硅胶、凝胶Sephadex LH-20、MCI、大孔吸附树脂等多种柱色谱技术分离得到单体化合物，根据其理化性质结合波谱学技术对其结构进行鉴定。采用MTT法检测化合物对转化生长因子-β1（transforming growth factor β1，TGF-β1）诱导BEAS-2B细胞损伤的保护作用，初步探讨其潜在抗肺纤维化活性。结果 从箭叶淫羊藿正丁醇部位分离得到了16个化合物，分别鉴定为5,7-二羟基-4′-甲氧基-黄酮-[3-O-α-L-鼠李糖基-(1→2)-α-L-鼠李糖苷]（1）、8-异戊烯基山柰酚（2）、淫羊藿次苷-Ⅰ（3）、sagittasine C（4）、5-hydroxy-4'-methoxy-8-(2-hydroxy-3-methyl-3-butenyl) flavone 3-O-α-L-rhamnopyranosyl-7-O-β-D-glucopyranoside（5）、anhydroicaritin-3-O-glucosyl 7-O-glucoside（6）、hexandraside E（7）、箭藿苷B（8）、sutchuenmedin B（9）、二叶淫羊藿苷B（10）、朝藿定C（11）、槲皮素（12）、金丝桃苷（13）、myricitrin（14）、(＋)-儿茶素（15）、(−)-表儿茶素（16）。结论 化合物1为新化合物，命名为箭藿苷D；化合物2、5～7、9、14～16均首次从箭叶淫羊藿中分离得到，其中化合物8和16能显著提高TGF-β1诱导的BEAS-2B损伤细胞的活力，具有潜在抗肺纤维化活性。

Objective To study the chemical constituents of n-butanol part of Epimedium sagittatum and their anti-pulmonary fibrosis activity. Method The chemical components were isolation and purification by modern column chromatography, and their structures were identified by spectrography techniques. The anti-pulmonary fibrosis activity of these compounds from E. sagittatum were evaluated by MTT on BEAS-2B cell damage induced by TGF-β1. Result Sixteen compounds were obtained from the E. sagittatum, which were identified as 5,7-dihydroxy-4′-methoxyflavone 3-O-α-L-rhamnosyl-(1→2)-α-L-rhamnoside​ (1), 8-prenylkaempferol (2), icariside I (3), sagittasine C (4), 5-hydroxy-4'-methoxy-8-(2-hydroxy-3-methyl-3-butenyl) flavone 3-O-α-L-rhamnopyranosyl-7-O-β-D-glucopyranoside (5), anhydroicaritin-3-O-glucosyl 7-O-glucoside (6), hexandraside E (7), sagittatoside B (8), sutchuenmedin B (9), diphylloside B (10), epimedin C (11), quercetin (12), hyperoside (13), myricitrin (14), (+)-catechin (15), (−)-epicatechin (16). Conclusion Compound 1 is a new compound, named epimedin D. Compounds 2, 5—7, 9, 14—16 were isolated from E. sagittatum for the first time, and compounds 7 and 15 significantly increased the viability of BEAS-2B cells induced by TGF-β1.

R284.1

国家重点研发计划（中医药现代化研究重大专项）（2019YFC1708800）；河南省自然科学基金资助项目（252300420701）；河南省高等学校重点科研项目（24A360017）