目的 分析1～12月忍冬Lonicerae japonicae叶中12种活性成分含量的动态变化，评价不同采收期忍冬叶质量。方法 采用UPLC法检测每月采摘的忍冬叶中绿原酸、当药苷、断氧化马钱子苷、异槲皮苷等12种活性成分的含量，绘制动态变化曲线，分析成分含量变化，并结合聚类分析、主成分分析（principal component analysis，PCA）、正交偏最小二乘判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA），构建不同采收期忍冬叶中12种活性成分的含量差异性模型，综合评价忍冬叶的质量。结果 UPLC法测得3、2月忍冬叶中化学成分的含量较高，4、12、1月次之。根据所测每月忍冬叶中活性成分的含量，聚类分析、PCA、OPLS-DA分析一致表明，忍冬叶被分为4类，12、1、2月为一类，5、6、7、8、9、10、11月为一类，3月为一类，4月为一类。进一步的多元统计分析显示，绿原酸、异绿原酸A、木犀草苷或是导致忍冬叶质量差异的关键成分。结论 忍冬叶中12种活性成分含量一年中变化显著，3、2、4、12、1月忍冬叶的质量较好，绿原酸、异绿原酸A、木犀草苷可以作为忍冬叶质控的关键成分。结合忍冬种植模式，12、1、2月为忍冬叶最佳采收期。研究结果为忍冬叶的质量控制及评价提供方法和依据，也为忍冬叶的合理开发利用提供支持。

Objective To evaluate the quality of Lonicerae japonicae leaves picked from January to December based on the dynamic changes of 12 active ingredients. Methods An ultra-high performance liquid chromatography (UPLC) method was used to determinethe contents of 12 active ingredients such as chlorogenic acid, sweroside, secoxyloganin and isoquercetin in Lonicerae japonicae leaves picked every month. Dynamic change curves were plotted to analyze the content change of each ingredient. To comprehensively evaluate the quality of Lonicerae japonicae leaves harvested at different periods, clustering analysis, principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were respectively constructed to compare the contents of 12 active components. Results The 12 active ingredients changed significantly throughout the year. The total contents of chemical components in Lonicerae japonicae leaves picked in March and February were the highest, followed by April, December and January. According to the content of active ingredients in Lonicerae japonicae leaves measured each month, the results of cluster analysis, PCA and OPLS-DA consistently indicated that Lonicerae japonicae leaves were divided into four categories, one in December, January and February, one in May, June, July, August, September, October and November, one in March and one in April. Further multivariate statistical analysis showed that chlorogenic acid, isochlorogenic acid A and luteolin might be the key components causing the quality difference of Lonicerae japonicae leaves. Conclusion The contents of 12 active ingredients in Lonicerae japonicae leaves picked in different months were significantly different. The quality of Lonicerae japonicae leaves was better in March, February, April, December and January. Chlorogenic acid, isochlorogenic acid A and luteolin could be used as key components for quality control of Lonicerae japonicae leaves. In combination with the planting pattern of Lonicerae japonicae, December, January and February were the best harvesting periods for the leaves. This study will provide methods and basis for the quality control and evaluation of the Lonicerae Japonicae leaves, and also offers support for their rational development and utilization.

R286.2

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