目的 探究不同采收期茵陈中化学成分的动态变化规律，为揭示“绵茵陈”和“花茵陈”品质形成机制奠定基础。方法 以6个不同采收期（S1：幼苗期、S2～S4：营养生长期、S5：开花期、S6：结果期）茵陈蒿为材料，利用HPLC和分光光度法对主要活性物质含量以及体外抗氧化能力进行测定与分析。结果 随着采收期延长，绿原酸含量呈显著下降趋势，在S1时期（“绵茵陈”期）最高5.69 mg/g，而滨蒿内酯含量呈显著增加趋势，在S5时期（“花茵陈”期）最高3.58 mg/g。多糖含量在S1和S5时期相对较高，酚类和黄酮类含量以及1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）清除率和铁离子还原/氧化能力（ferric reducing/antioxidant power，FRAP）值均呈“M型”变化趋势，表现S2、S3和S5时期相对较高。结论 采收期对茵陈中化学成分具有显著影响，为茵陈资源开发和利用提供理论依据和有益参考。

Objective To explore the dynamic change rule of chemical compounds in Artemisia capillaris at different harvest stages, and lay the foundation for revealing the formation mechanism of quality in A. capillaris harvested in spring and autumn. Methods The main active substance contents and in vitro antioxidant capacity in A. capillaris harvested at six different stages (S1: seedling stage, S2 to S4: vegetative growth stage; S5: flowering stage; S6: fruiting stage) were determined and analyzed using the HPLC and spectrophotometry. Results With the extension of harvest stage, the chlorogenic acid contents showed a significant decrease and reached the highest value of 5.69 mg/g at the S1 (seedling stage), while the scoparone content showed a significant increase and reached the highest value of 3.58 mg/g at the S5 (flower bud stage). The polysaccharide contents were relatively high at S1 and S5, and the phenolic and flavonoids and phenolic contents as well as DPPH scavenging rate and FRAP value showed a “M” changetrend with a relatively high l level at the S2、S3 and S5. Conclusion There is a significant effect of harvest stage on chemical compounds in A. capillaris, these findings will provide theoretical basis and useful references for resource exploitation and utilization of A. capillaris.

R286.2

国家中药材产业技术体系（CARS-21）；道地药材生态种植及质量保障项目（202203002）