目的 利用光照处理、低温处理与赤霉素A3（gibberellic acid，GA3）处理探究月见草Oenothera parviflora抽薹的影响因素，通过转录组筛选GA3处理条件下的抽薹开花相关基因。方法 分别利用0、200、400、600、800 mg/L GA3处理3 d和10 d，并测定月见草的的抽薹率，进一步对喷施GA3前后月见草的茎尖进行转录组分析，筛选抽薹开花相关差异表达基因。结果 发现，仅有GA3处理月见草营养体的抽薹效果最为显著，其中，600 mg/L GA3处理条件下月见草的抽薹率最高，抽薹率为96.67%。对其进行转录组分析发现，GA3喷施后有1 626个基因差异表达，其中有863个基因表达上调，763个基因显著下调。通过基因注释信息与拟南芥同源基因比较发现，抽薹开花相关基因OpTEM1、OpCO、OpFD、OpAGL19、OpAGL42、OpAP1、OpRGL1、OpSPL5可能在GA3作用下参与调控月见草抽薹开花调控。利用荧光定量进一步确认差异基因的表达模式，其中ObCO、ObAP1、ObAGL19、ObAGL42、ObSPL5基因显著上调，ObTEM1、ObFD、ObRGL1基因显著下调，结果与转录组一致。结论 外源GA3可以显著促进月见草的抽薹开花进程，TEM1、CO、FD、AGL19、AGL42、AP1、RGL1、SPL5等基因可能参与调控月见草的抽薹开花进程。可以揭示月见草植物抽薹特性及其植株发育动态特征，对进一步明确月见草的抽薹机制和指导月见草生产实践具有重要意义。

Objective The influencing factors of bolting in Oenothera parviflora were investigated by light treatment, low-temperature treatment and gibberellic acid (GA3), and the genes related to bolting and flowering under GA3 treatment were screened by transcriptome. Method Different concentrations of GA3 with 0, 200, 400, 600, 800 mg/L were applied for treatment 3 d and 10 d, respectively, and the bolting rate of Oenothera parviflora were determined. Further transcriptome analysis was conducted on the stem apex before and after GA3 spraying to screen for differentially expressed genes related to bolting and flowering. Results varying photoperiod, light quality, low temperature, and treatments with GA3 indicated that GA3 application had the most significant impact on the bolting time. Among the different treatments, 600 mg/L GA3 solution led to the highest bolting rate at 96.67%. Transcriptional profiling showed that following GA3 treatment, 1 626 genes demonstrated differential expression, with 863 upregulated and 763 downregulated genes. Compared to homologous genes in Arabidopsis thaliana, suggested that genes such as OpTEM1, OpCO, OpFD, OpAGL19, OpAGL42, OpAP1, OpRGL1, OpSPL5, which are related to bolting and flowering, might play a role in regulating bolting and flowering time under GA3 treatment. Conclusion These findings shed light on the bolting characteristics and developmental dynamics of the Oenothera parviflora, which is crucial for elucidating the bolting mechanisms and guiding practical cultivation strategies.

R286.2

国家现代农业产业技术体系辽宁创新团队建设