目的 采用谱效关联分析探究八角茴香挥发油抗代谢相关脂肪性肝病（metabolic dysfunction-associated fatty liver disease，MAFLD）的质量标志物（quality marker，Q-Marker）。方法 采用GC-MS法建立15批八角茴香挥发油指纹图谱，并进行聚类分析；采用4.0%果糖诱导斑马鱼MAFLD模型评价八角茴香挥发油抗MAFLD的药效作用；灰色关联度分析探讨谱效相关性。结果 建立的八角茴香挥发油GC-MS指纹图谱标定10个共有峰，相似度为0.995～1.000。聚类分析将样品分为4类。与模型组比较，不同产地八角茴香挥发油对MAFLD模型斑马鱼的肝脏脂质积累、丙氨酸氨基转移酶（alanine aminotransferase，ALT）、天门冬氨酸氨基转移酶（aspartate aminotransferase，AST）、总胆固醇（total cholesterol，TC）、三酰甘油（triglycerides，TG）改善效果不同，大部分批次具有显著改善作用。灰色关联度分析显示，10个共有峰与5项药效指标的关联度值多数在0.6以上，呈现整体关联性。谱效关系分析预测，反式茴香脑、α-香柠檬烯、松油烯-4-醇等多成分协同抗MAFLD，可作为其潜在Q-Marker。结论 通过谱效关系分析发现反式茴香脑、α-香柠檬烯、松油烯-4-醇可作为八角茴香挥发油的潜在Q-Marker。

Objective To explore the quality markers (Q-Markers) of Bajiaohuixiang (Anisi Stellati Fructus) essential oils in treatment of metabolic dysfunction-associated fatty liver disease (MAFLD) using spectral effect correlation analysis. Methods GC-MS was used to establish 15 batches of fingerprint spectra of Anisi Stellati Fructus essential oils and cluster analysis was conducted. The pharmacological effect of Anisi Stellati Fructus essential oils on MAFLD induced by 4.0% fructose in zebrafish model were evaluated. Grey correlation analysis was used to explore the correlation between spectrum effects. Results The GC-MS fingerprint spectrum of Anisi Stellati Fructus essential oils was calibrated with 10 common peaks, and the similarity ranged from 0.995 to 1.000. Cluster analysis divides the samples into four categories. Compared with model group, Anisi Stellati Fructus essential oils from different origins had different improvement effects on liver lipid accumulation, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC) and triglycerides (TG) in zebrafish with MAFLD model, of which most batches had significant improvement effects. The grey correlation analysis showed that the correlation values between the 10 common peaks and five pharmacological indicators were mostly above 0.6, indicating an overall correlation. Spectral effect relationship analysis predicted that multiple components such as trans-anethole, α-bergamotene and terpinene-4-ol could synergistically resist MAFLD and serve as potential Q-Markers. Conclusion Trans-anethole, α-bergamotene and terpinene-4-ol can serve as potential Q-Markers for Anisi Stellati Fructus essential oils through spectral effect relationship analysis.

R282.6

广西中医药大学大学生创新训练项目（S202510600077）；广西青苗人才资助科研项目；引进博士科研启动基金项目（2022BS022）；广西中药药效研究重点实验室（省级）系统性研究课题（20-065-38-A3）