目的 建立不同产地狭叶薰衣草Lavandula angustifolia的HPLC指纹图谱及多成分含量测定方法，结合化学模式识别法评价狭叶薰衣草的质量，为其进一步研究和开发提供依据。方法 采用ZORBAX Eclipse XDB-C₁₈色谱柱（250 mm×4.6 mm，5 μm），乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，检测波长350 nm，柱温30 ℃，体积流量0.6 mL/min，进样量10 μL，建立新疆产地狭叶薰衣草HPLC指纹图谱并分析相似度，结合对照品对比指认化学成分并进行定量测定。通过Origin2025b、IBM SPSS Statistics 27和SIMCA 14.1软件进行聚类分析（hierarchical clustering analysis，HCA）、主成分分析（principal component analysis，PCA）及正交偏最小二乘法判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA），对不同产地狭叶薰衣草进行质量评价，筛选差异性标志物。结果 建立了不同产地狭叶薰衣草的HPLC指纹图谱，共标定14个共有色谱峰，指认出其中5个成分，分别为绿原酸（峰2）、木犀草苷（峰8）、迷迭香酸（峰12）、木犀草素（峰13）、芹菜素（峰14）；15批样品相似度均在0.9以上；HCA将15批狭叶薰衣草分为3类；PCA提取了4个主成分，其方差累积贡献率为93.310%；PCA结果显示新疆霍城样品（批号YC23072402）质量最优；OPLS-DA筛选出狭叶薰衣草样品中木犀草素、迷迭香酸和芹菜素等7个质量差异标志物，狭叶薰衣草中木犀草素、迷迭香酸、芹菜素质量分数分别为0.018～0.230、0.380～3.095、0.144～0.481 mg/g。结论 建立的新疆不同产地狭叶薰衣草HPLC指纹图谱及多成分含量测定方法简单、准确、重复性好，可用于狭叶薰衣草的质量评价，为其质量控制提供依据。

Objective To establish the HPLC fingerprint and multi-component content determination method of Lavandula angustifolia from different origins, and to evaluate the quality of L. angustifolia medicinal materials from different origins by combining chemical pattern recognition methods, providing a basis for further research and development. Methods A Zorbax Eclipse XDB-C₁₈ column (250 mm × 4.6 mm, 5 μm) was used, with acetonitrile-0.1% phosphoric acid solution as the mobile phase, and gradient elution. The detection wavelength was 350 nm, the column temperature was 30 ℃, the flow rate was 0.6 mL/min, and the injection volume was 10 μL. The HPLC fingerprint of L. angustifolia from Xinjiang was established and the similarity was analyzed. At the same time, the chemical components were identified by comparison with reference substances and quantitatively determined. Hierarchical clustering analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA) were performed using Origin2025b, IBM SPSS Statistics 27 and SIMCA 14.1 software to evaluate the quality of L. angustifolia medicinal materials from different origins and screen the quality difference markers. Results The HPLC fingerprint chromatograms of L.angustifolia from different regions in Xinjiang were established. A total of 14 common chromatographic peaks were identified, and five components were recognized: Chlorogenic acid (peak 2), luteolin-7-O-glucoside (peak 8), rosmarinic acid (peak 12), luteolin (peak 13), and apigenin (peak 14).The similarity of 15 batches of samples was all above 0.9. HCA divided 15 batches of L. angustifolia into three categories. PCA extracted four principal components, with a cumulative contribution rate of 93.310%. PCA results showed that batch YC23072402 from Huocheng, Xinjiang, had the best quality. OPLS-DA identified seven quality difference markers, namely luteolin, rosmarinic acid and apigenin, in L. angustifolia samples. The contents of luteolin, rosmarinic acid and apigenin in L. angustifolia were 0.018—0.230, 0.380—3.095 and 0.144—0.481 mg/g respectively. Conclusion The HPLC fingerprint and multi-component content determination method for L. angustifolia from different origins is simple, accurate, and has good repeatability. It can be used for the quality evaluation of L. angustifolia from Xinjiang and provide a basis for its quality control.

R282.6

河南省杰出青年基金项目（252300421028）；河南省高校科技创新团队（26IRTSTHN018）