目的 剖析蛇莓Duchesnea indica不同组织内生细菌群落的多样性及其功能，揭示内生细菌与蛇莓药用活性间的潜在联系。方法 以蛇莓组织根、茎、叶、果中内生细菌为研究对象，运用Illumina高通量技术对群落DNA片段进行双端测序，借助DADA2和Vsearch方法处理序列，进行生物信息学分析及功能潜能预测。结果 蛇莓内生细菌基因组经高通量测序，共获取1 914 360条原始序列，在“门”水平上，以变形菌门（Proteobacteria）为优势菌群。不同组织中内生细菌以茎内丰富度最高，根内多样性最高。鞘氨醇单胞菌属Sphingomonas是区分组间差异的关键标志物种，与假单胞菌属Pseudomonas呈显著正相关，且二者是N₁₀-甲酰基四氢叶酸生物合成代谢通路的主要构成菌属。结论 蛇莓内生细菌群落在叶、果实与茎中相似性较高，与根差异明显；假单胞菌属与鞘氨醇单胞菌可能在次生代谢产物合成或宿主抗逆性增强中发挥协同作用。

Objective To analyze the diversity and functional potential of endophytic bacterial communities in different tissues of Duchesnea indica and explore their potential relationship with the medicinal activity of the host plant. Methods Endophytic bacteria from the root, stem, leaf and fruit tissues of D. indica were studied. DNA fragments of the microbial communities were subjected to paired-end sequencing using Illumina high-throughput technology. Sequences were processed using DADA2 and Vsearch, followed by bioinformatics analysis and functional potential prediction. Results High-throughput sequencing of the endophytic bacterial genomes yielded a total of 1 914 360 raw sequences. At the phylum level, Proteobacteria was the dominant group. Among different tissues, the stem exhibited the highest richness of endophytic bacteria, while the root showed the highest diversity. Sphingomonas was identified as a key biomarker distinguishing intergroup differences and showed a significant positive correlation with Pseudomonas. Both bacterial genuses were major contributors to the N₁₀-formyltetrahydrofolate biosynthesis pathway. Conclusion The endophytic bacterial communities in the leaf, fruit and stem tissues of D. indica exhibited high similarity but were significantly distinct from those in the root. Pseudomonas and Sphingomonas may play synergistic roles in the synthesis of secondary metabolites or in enhancing host stress resistance.

R282.12

浙江省尖兵领雁研发攻关计划项目（2023C02042）