目的 研究裸花紫珠Callicarpa nudiflora干燥叶的化学成分，挖掘其抗耐甲氧西林金黄色葡萄球菌（methicillin-resistance Staphylococcus aureus，MRSA）活性成分。方法 利用硅胶柱色谱、凝胶、ODS柱色谱等色谱技术方法进行分离纯化，并综合运用IR、HR-ESI-MS、¹H-NMR、¹³C-NMR、HSQC、HMBC、COSY等方法鉴定化合物的结构，采用营养肉汤稀释法测定化合物对金黄色葡萄球菌、MRSA、大肠杆菌、多重耐药大肠杆菌的最低抑菌浓度。结果 从裸花紫珠乙醇提取物醋酸乙酯萃取部位分离得到1个单萜类化合物（1）和4个倍半萜类化合物（2～5），分别鉴定为 (Z)-2-甲基-6-亚甲基辛-2,7-二烯-1-基(E)-3-(4-羟基苯基)丙烯酸酯（1）、monasuslunin（2）、(1S,5R,9R)-10,10-二甲基-2,6-二亚甲基双环[7.2.0]十一烷-5-醇（3）、7-表-桉叶-4(15)-烯-1β,6β-二醇（4）、香紫苏内酯（5），其中化合物1对金黄色葡萄球菌29213和MRSA T144表现出较强的抑制作用，最低抑菌浓度（minimum inhibitory concentrations，MIC）均为8 μg/mL，对大肠杆菌25922、大肠杆菌B2的MIC均大于8 μg/mL。结论 化合物1为新化合物，命名为紫珠烯A；化合物3～5为属内首次分离得到，化合物1具有较强的的抗MRSA活性。

Objective To study the chemical constituents of the dried leaves of Callicarpa nudiflora and discover bioactive compounds with anti-methicillin-resistance Staphylococcus aureus (MRSA) effects. Methods A systematic separation of chemical constituents was conducted by comprehensive chromatographic methods, including silica gel, Sephadex LH-20, ODS column chromatography and other chromatographic techniques. The structures of the isolated compounds were determined on the basis of spectroscopic analysis including IR, HR-ESI-MS, ¹H-NMR, ¹³C-NMR, HSQC, HMBC, COSY, etc. The minimum inhibitory concentrations (MICs) of the compounds against Staphylococcus aureus, MRSA, Escherichia coli, and multidrug-resistant E. coli were determined by the nutrient broth dilution method. Results One monoterpene, named (Z)-2-methyl-6-methyleneocta-2,7-dine-1-yl (E)-3-(4-hydroxyphenyl)acrylate (1), and four sesquiterpenoids, named monasuslunin (2), (1S,5R,9R)-10,10-dimethyl-2,6-dimethylenebicyclo [7.2.0] undecan-5-ol (3), 7-epi-eudesm-4(15)-ene-1β,6β-diol (4), sclareolide (5) were isolated and identified. Compound 1 exhibited strong inhibitory effects against S. aureus 29213 and MRSA T144, with MICs of 8 μg/mL for both. The MICs against E. coli 25922 and E. coli B2 were both greater than 8 μg/mL. Conclusion Compound 1 is a new compound named Callinuene A and compounds 3-5 are first isolated within this species. Compound 1 exhibits significant anti-MRSA activity.

R284.1

国家自然科学基金资助项目（81903481）；江苏省研究生科研创新计划（KYCX24_3844）；扬州大学2025年大学生创新创业训练计划项目（XCX20250849）