目的 基于多指标含量测定，采用主成分分析（principal component analysis，PCA）、偏最小二乘-判别分析（partial least squares-discriminant analysis，PLS-DA）及CRITIC-TOPSIS法评价不同产地黄姜花Hedychium flavum质量差异，为进一步开发利用提供依据。方法 采用HPLC法，BetaMax Neutral C₁₈色谱柱，乙腈-0.4%磷酸水溶液梯度洗脱；采集不同产地17批黄姜花，检测原儿茶酸、对香豆酸、阿魏醛、异姜花素D、姜花素D、姜花素A、姜花素E、槲皮素、山柰酚、异鼠李素、胡萝卜苷、豆甾醇、β-谷甾醇含量，并检查浸出物和总灰分，以PCA和PLS-DA方法对17批黄姜花进行分组，筛选质量差异标志物；以CRITIC法计算各指标权重，采用TOPSIS法构建黄姜花综合质量评价模型。结果 17批黄姜花中以上15个指标含量分别为0.121～0.295、0.044～0.091、0.036～0.104、0.400～1.071、0.300～0.515、0.902～1.677、0.089～0.153、0.473～0.916、0.177～0.378、0.136～0.249、0.068～0.117、0.223～0.453、0.240～0.863 mg/g，11.4%～21.5%、3.1%～9.2%。PCA分析得2个主成分的累积方差贡献率为87.564%，并将17批黄姜花分为3类；PLS-DA分析筛选出山柰酚、姜花素A、原儿茶酸、浸出物、β-谷甾醇、异姜花素D和槲皮素为质量差异标志物；CRITIC-TOPSIS法结果显示17批黄姜花样品的综合相对接近度为0.421 6～0.618 1，贵州和四川产地样品质量较优。结论 所建立的多指标定量结合PCA、PLS-DA及CRITIC-TOPSIS法可综合评价不同产地黄姜花药材质量差异，且该方法科学直观、准确可靠，可为黄姜花的质量差异评价提供参考。

Objective To evaluate the quality difference evaluation of Hedychium flavum from different origins based on multi-index content determination, using principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (PLS-DA) and CRITIC-TOPSIS methods, so as to provide a basis for further development and utilization. Methods HPLC was used with BetaMax Neutral C₁₈ column, acetonitrile -0.4%phosphoric acid solution as mobile phase with gradient elution, detection wavelength of 320, 230, 360 and 210 nm. A total of 17 batches of H. flavum from different origins were collected. The contents of 13 chemical constituents including protocatechuic acid, trans-4-hydroxycinnamic acid, coniferaldehyde, isocoronarin D, coronarin D, coronarin A, coronarin E, quercetin, kaempferol, isorhamnetin, daucosterol, stigmasterol, β-sitosterol were detected, while extractives and total ash were examined. The 17 batches of H. flavum were grouped using PCA and PLS-DA methods, and quality differential markers were screened out. The weights of each index was calculated using the CRITIC method, and a comprehensive quality evaluation model for H. flavum was constructed using the TOPSIS method. Results The contents of the above 13 indexes among 17 batches of H. flavum were 0.121—0.295, 0.044—0.091, 0.036—0.104, 0.400—1.071, 0.300—0.515, 0.902—1.677, 0.089—0.153, 0.473—0.916, 0.177—0.378, 0.136—0.249, 0.068—0.117, 0.223—0.453, 0.240—0.863 mg/g, while the ranges of extractives and total ash were 11.4%—21.5% and 3.1%—9.2 %, respectively. PCA results showed that the cumulative variance contribution rate of two principal components was 87.564%, and 17 batches of H. flavum were divided into three categories. Kaempferol, coronarin A, protocatechuic acid, extractives, β-sitosterol, isocoronarin D and quercetin were selected as quality differential markers by PLS-DA methods. The results of CRITIC-TOPSIS method showed that the comprehensive relative proximities of 17 batches of H. flavum samples was 0.421 6—0.618 1. The quality of samples from Guizhou and Sichuan provinces exhibited superior comprehensive quality. Conclusion The established multi-index quantitative combined with PCA, PLS-DA and CRITIC-TOPSIS method can comprehensively evaluate the quality difference of H. flavum from different habitats. The method is scientific, intuitive, accurate and reliable, which can provide a theoretical and technical reference for the quality difference evaluation of H. flavum.

R282.6

海南省药物警戒创新研究课题（HNPV20）