目的 研究杭白芷Angelica dahurica var. formosana中微量的呋喃香豆素二聚体类化学成分。方法 以LC-MS分析为导向，利用硅胶柱色谱、葡聚糖凝胶柱色谱及半制备液相色谱等进行分离纯化，结合NMR等谱学技术对分离得到的香豆素二聚体进行结构鉴定，并对分离得到的化合物进行乙酰胆碱酯酶抑制活性筛选。结果 从杭白芷乙醇提取物的醋酸乙酯萃取部位分离得到3个香豆素二聚体，分别为9-{[4-(3-甲基丁-2-烯-1-基)-7-氧代-7H-呋喃并[3,2-g]色烯-9-基]氧基}-4-[(2-甲基丁-3-烯-2-基)氧基]-7H-呋喃并[3,2-g]色烯-7-酮（1）、dahuribiethrin E（2）、dahuribiethrin B（3）。结论 化合物1为新化合物，命名为白芷双香豆素A；化合物2和3为首次从杭白芷中分离得到，化合物2对乙酰胆碱酯酶具有抑制活性，半数抑制浓度（median inhibition concentration，IC₅₀）值为（71.37±1.26）µmol/L。

Objective To investigate the trace amounts of furanocoumarin dimers from Angelica dahurica var. formosana. Methods Using an LC-MS guided separation method, the furanocoumarin dimers were isolated by silica gel, Sephadex LH-20 column chromatography, and semi-preparative HPLC. The structures of the isolated dimeric coumarins were identified by NMR spectroscopy, and their preliminarily acetylcholinesterase (AChE) inhibitory activities were evaluated. Results Three furanocoumarin dimers were isolated from the ethyl acetate fraction of the ethanol extract of A. dahurica var. formosana, namely 9-{[4-(3-methylbut-2-en-1-yl)-7-oxo-7H-furo[3,2-g]chromen-9-yl]oxy}-4-[(2-methylbut-3-en-2-yl)oxy]-7H-furo[3,2-g]chromen-7-one (1), dahuribiethrin E (2) and dahuribiethrin B (3). Conclusion Compound 1 is a new compound, namely dahuribiscoumarin A. Compounds 2 and 3 are isolated from A. dahurica var. formosana for the first time. Compound 2 exhibits acetylcholinesterase inhibitory activity with a median inhibition concentration (IC₅₀) value of (71.37 ± 1.26) μmol/L.

R284.1

北京市自然科学基金资助项目（7202125）