目的 对不同产地千年健Homalomena occulta中多指标含量进行检测比较，为千年健质量评价提供依据。方法 采集19批不同产地千年健样品，采用HPLC法，以CenturySIL C₁₈BDS柱（250 mm×4.6 mm，5 μm）为分离色谱柱，乙腈-0.3%磷酸为流动相梯度洗脱，波长切换（260、330、210 nm），同步检测千年健中原儿茶酸、咖啡酸、阿魏酸、槲皮素、芹菜素、乔松素、胡萝卜苷、β-谷甾醇、豆甾醇、熊果酸、桦木酸11个成分的含量；同时参照《中国药典》2025年版方法测定芳樟醇含量，并检查醇溶性浸出物、总灰分和酸不溶性灰分。结合聚类分析（hierarchical cluster analysis，HCA）、主成分分析（principal component analysis，PCA）、因子分析（factor analysis，FA）、偏最小二乘法-判别分析（partial least squares-discriminant analysis，PLS-DA）及灰色关联度分析（grey relational analysis，GRA）对不同产地千年健的质量进行综合评价与差异标志物筛选。结果 19批千年健中各指标质量分数分别为原儿茶酸0.251～0.903 mg/g、咖啡酸0.171～0.294 mg/g、阿魏酸0.041～0.101 mg/g、槲皮素0.265～0.490 mg/g、芹菜素0.144～0.250 mg/g、乔松素0.046～0.088 mg/g、胡萝卜苷0.237～0.509 mg/g、β-谷甾醇0.394～1.153 mg/g、豆甾醇0.102～0.218 mg/g、熊果酸0.154～0.328 mg/g、桦木酸0.071～0.116 mg/g、芳樟醇3.022～5.079 mg/g、浸出物19.5%～33.5%、总灰分2.4%～6.5%和酸不溶性灰分0.5%～1.2%。化学模式识别分析表明，不同产地千年健质量存在一定的差异性，19批样品聚为3组，产地相近的样品为一组，区分各样品的质量差异标志物为芳樟醇、槲皮素、熊果酸、β-谷甾醇和原儿茶酸。FA与GRA法的质量评价结果一致，广西产S15～S19样品质量较优。结论 通过分析比较不同产地千年健中多指标含量，确定区分各样品的质量差异标志物，为千年健的质量评价提供方法参考。

Objective To detect and compare the contents of multiple indexes in Homalomena occulta from different producing areas, and to provide basis for quality evaluation of H. occulta. Methods In this study, 19 batches of H. occulta were collected. An HPLC method was performed on a CenturySIL C₁₈ BDS column (250 mm × 4.6 mm, 5 μm) with acetonitrile-0.3% phosphoric acid as the mobile phase under gradient elution and wavelength switching detection at 260, 330 and 210 nm to simultaneously determine the contents of 11 components (protocatechuic acid, caffeic acid, ferulic acid, quercetin, apigenin, pinocembrin, daucosterol, β- sitosterol, stigmasterol, ursolic acid, betulinic acid). The content of linalool was determined, and the alcohol-soluble extract, total ash and acid-insoluble ash were examined according to the 2025 edition of Chinese Pharmacopoeia. Hierarchical cluster analysis, principal component analysis (PCA), factor analysis (FA), partial least squares discriminant analysis (PLS-DA) and grey relational analysis (GRA) were comprehensively applied to evaluate the quality of H. occulta from different producing areas and to screen the quality difference markers. Results The contents of each index in 19 batches of H. occulta were protocatechuic acid 0.251—0.903 mg/g, caffeic acid 0.171—0.294 mg/g, ferulic acid 0.041—0.101 mg/g, quercetin 0.265—0.490 mg/g, apigenin 0.144—0.250 mg/g, pinocembrin 0.046-0.088 mg/g, daucosterol 0.237—0.509 mg/g, β-sitosterol 0.394—1.153 mg/g, stigmasterol 0.102—0.218 mg/g, ursolic acid 0.154—0.328 mg/g, betulinic acid 0.071—0.116 mg/g, linalool 3.022—5.079 mg/g, extract 19.5%—33.5%, total ash 2.4%—6.5% and acid-insoluble ash 0.5%—1.2%. Chemical pattern recognition analysis showed that there were some differences in the quality of H. occulta in different producing areas. The 19 batches of samples were divided into 3 groups, and the samples with similar origin were divided into one group. The quality difference markers of each sample were linalool, quercetin, ursolic acid, β-sitosterol and protocatechuic acid. The quality evaluation results of factor analysis and grey correlation analysis were consistent, which showed that the quality of S15—S19 samples from Guangxi was better. Conclusion By analyzing and comparing the contents of multiple indexes in H. occulta from different producing areas, the quality difference markers for distinguishing each sample were determined, which can provide a method reference for the quality evaluation of H. occulta.

R282.6

安徽省高等学校科学研究项目（2024AH051978）