目的 为明确蒲公英Taraxacum mongolicum不同部位间化学成分差异，以蒲公英栽培品和野生品为研究对象，将其分为全草、地上部分、根3个部位进行比较。方法 采用Venusil C₁₈色谱柱以乙腈-0.2%磷酸，流动相梯度洗脱，体积流量1.0 mL/min，柱温30 ℃，检测波长330 nm。建立蒲公英药材的指纹图谱，测定不同部位蒲公英的6个成分（单咖啡酰酒石酸、绿原酸、咖啡酸、阿魏酸、异绿原酸C、菊苣酸）含量，结合相似度评价、聚类分析（cluster analysis，CA）和正交偏最小二乘判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）对采集的指纹图谱信息进行分析。结果 蒲公英指纹图谱标定9个共有峰，并确认其中6个主要化学成分，筛选5个主要差异性成分。定量分析结果显示，蒲公英根相比较蒲公英全草和地上部分，化学成分含量差异较大。结论 该方法简便可靠，重复性好，建立的综合质量评价模型的分析结果全面客观，可用于蒲公英药材整体质量的综合评价，丰富了蒲公英有关质量分析的研究，为蒲公英以后开发、质量控制和临床应用提供了依据和参考。

Objective To elucidate the chemical composition differences among various parts of Pugongying (Taraxacum mongolicum), this study compared cultivated and wild T. mongolicum samples, dividing them into three parts: whole plant, above-ground parts, and roots. Methods A Venusil C₁₈chromatographic column was used with acetonitrile-0.2% phosphoric acid as the mobile phase for gradient elution at a flow rate of 1.0 mL/min, column temperature of 30 °C, and detection wavelength of 330 nm. A fingerprint spectrum of T. mongolicum medicinal materials was established, and the contents of six components (monocaffeoyl tartaric acid, chlorogenic acid, caffeic acid, ferulic acid, isochlorogenic acid C, and chicoric acid) were determined in different parts. The collected fingerprint spectrum data were analyzed using similarity evaluation, cluster analysis (CA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Results The T. mongolicum fingerprint spectrum identified nine common peaks, with six major chemical components confirmed and five primary differential components screened. Quantitative analysis revealed significant differences in chemical composition content between T. mongolicum roots compared to whole plant and above-ground parts. Conclusion This method is simple, reliable, and highly reproducible. The comprehensive quality evaluation model provides objective analytical results suitable for holistic quality assessment of T. mongolicum medicinal materials. This study enriches research on quality analysis of T. mongolicum and offers evidence for future development, quality standardization, and clinical application.

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